Primer, probe and kit for detecting microorganism in urogenital canal and use method of kit

A technology for microbial detection and genitourinary tract, which is applied in the field of molecular biology detection, can solve the problems of poor detection effect of pathogenic microorganisms, cumbersome processing process, poor specificity, etc., and achieve the advantages of avoiding PCR false negatives, high sensitivity and wide detection range Effect

Pending Publication Date: 2018-04-20
SANSURE BIOTECH
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Problems solved by technology

[0007] At present, there are some kit products based on real-time fluorescent quantitative PCR technology to detect Chlamydia trachomatis, Ureaplasma urealyticum or Neisseria gonorrhoeae DNA, but there are generally problems of poor specificity and low sensitiv

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[0031] In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the related drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that a thorough and complete understanding of the present disclosure is provided.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed ite

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Abstract

The invention discloses a primer, a probe and a kit for detecting the microorganism in a urogenital canal and a use method of the kit. By the primer, the probe and the kit for detecting the microorganism in the urogenital canal and the use method of the kit, the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus can be detected simultaneously, the extraction of the nucleic acid is only conducted and only one step of the PCR detection reaction is conducted through the real-time fluorescence PCR amplification technique, the use method of the kit is simple and rapid, the specificity is good, the pathogene microorganisms of the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus are not detected, the problem of false positive result is not easy to cause, thesensitivity is as high as 400 copies/ml, the detection range is 400 copies/ml to 4.00E+09 copies/ml, and the detection range is wide.

Description

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Claims

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Application Information

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Owner SANSURE BIOTECH
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