Method for quantitatively determining chlorogenic acid and geniposide in herba artemisiae scopariae detoxifying particles simultaneously

A technology for the quantitative determination of geniposide, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of reduced number of theoretical pedals, shortened service life, and random curling of chromatographic columns, and achieves wide practical range, low cost, and low instrument cost Reduced effect

Inactive Publication Date: 2018-12-21
ZHEJIANG DKCOM ANIMAL HEALTH PROD
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Problems solved by technology

The biggest defect of this mobile phase is: the concentration of phosphoric acid 0.4% is too high, and its acidity is below pH2 (the author once precisely measured the pH value of 0.1% phosphoric acid to be about 2.1), which does not meet the mobile phase pH value specified in the Chinese Pharmaco

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  • Method for quantitatively determining chlorogenic acid and geniposide in herba artemisiae scopariae detoxifying particles simultaneously
  • Method for quantitatively determining chlorogenic acid and geniposide in herba artemisiae scopariae detoxifying particles simultaneously
  • Method for quantitatively determining chlorogenic acid and geniposide in herba artemisiae scopariae detoxifying particles simultaneously

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[0034] Example

[0035] A. Chromatographic conditions and system suitability test Use octadecylsilane-bonded silica gel as filler; use acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 11:8:81 as mobile phase; detection wavelength: 235nm; column temperature It is 30℃; the number of theoretical plates is not less than 3000 according to the peak of geniposide;

[0036] B. Preparation of reference substance solution Weigh appropriate amount of chlorogenic acid and geniposide reference substance, add 70% methanol to make a solution containing about 0.015mg of chlorogenic acid and 0.026mg of geniposide per 1mL, as the reference solution;

[0037] C. Preparation of the test solution: Take an appropriate amount of Yinchen Jiedu granules, grind it into fine pieces, take about 0.4~0.6g, accurately weigh it, add 70% methanol 25mL, weigh it, and ultrasonic treatment (power 250W, frequency 33kHz) 15 After 10 minutes, take it off, weigh it again, use methanol to make up the lost wei

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Abstract

The invention relates to a method for quantitatively determining chlorogenic acid and geniposide in herba artemisiae scopariae detoxifying particles simultaneously. The method has the characteristicsthat a test solution is obtained by using a simple and fast pretreatment method; the content of chlorogenic acid and geniposide in a sample is determined at the wavelength of 235+/-1nm at the same time by adopting an ordinary chromatographic column and an isocratic elution method and taking acetonitrile-methanol-0. 1% phosphoric acid at the volume ratio of 11:8:91 as a mobile phase; wave peak separation is good; the peak area of two peaks is proper; the wave peak retention time of the chlorogenic acid is about 9min; the retention time of the geniposide is about 11min; and peak appearance is completed within 12min through one sample injection. The method has the beneficial effects that quantitative index doubling and supervision improvement are achieved, but the detection time is not prolonged and the detection cost is not increased. The method is simple, fast, accurate, low in cost, wide in practical range and easy to popularize and grasp.

Description

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Claims

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Application Information

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Owner ZHEJIANG DKCOM ANIMAL HEALTH PROD
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