Method for measuring content of L-carnitine in infant foods and dairy products by spectrophotometry

An infant food and spectrophotometry technique is applied in the field of determination of L-carnitine content in infant food and dairy products, which can solve the problems of complex sample processing, expensive equipment, and difficulty in universal application, and achieves simple operation and popularization of instruments. , the result is accurate

Inactive Publication Date: 2019-05-14
ZHENJIANG XIANCHUANG BIOTECH CO LTD
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Benefits of technology

The present technology described by this patented method allows scientists to accurately measure how much certain chemical compounds are found inside milk or baby's diet compared to other methods like spectrometry. It makes things simpler than previous techniques that were used beforehand because there was no complicated process involved during analysis.

Problems solved by technology

This patented method involves analyzing samples from cow's milk by measuring its contents with specific chemical compounds called caffeine or lysophospholysis glycerin (LPC). These techniques can provide important insights into how much carnitine has been consumed during pregnancy and affect their growth prospects.

Method used

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  • Method for measuring content of L-carnitine in infant foods and dairy products by spectrophotometry

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Experimental program
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specific Embodiment approach

[0012] 1. Instruments, reagents and materials:

[0013] instrument:

[0014] PH meter (accurate Swiss Mettler company); spectrophotometer (General Analysis General, T6 Xinyue)

[0015] Reagents and materials:

[0016] 13% perchloric acid; 10mol / L sodium hydroxide solution; 4.0mol / L potassium hydroxide solution; color stock solution: weigh 50mg of 2-ylbenzoic acid, 5.96g of N-2-N-2-alkanesulfonic acid, Dissolve 185 mg of disodium edetate dihydrate in 30 mL of deionized water, adjust the pH to 7.4-7.6 with 1.0 mol / L NaOH, and then dilute to 50 mL with water. This solution can be stored in a refrigerator at 4°C for 3 months. Chromogenic solution: Take 5.0mL of chromogenic stock solution and dilute to 25mL with water. Acetyl-CoA solution: Weigh 20.0mg of acetyl-CoA and dissolve it in 2.0mL of water (preparate now). Carnitine acetyltransferase (80U / mg; CAT) solution: 100μL of CAT suspension, centrifuged at 1500rpm for 10min, discarded the supernatant, and dissolved the pre

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Abstract

The invention discloses a method for measuring the content of L-carnitine in infant foods and dairy products by spectrophotometry. According to the research, by utilizing a principle that free L-carnitine in the products can react with acetylcoenzyme to generate acetylcarnitine and free coenzyme, the amount of yellow substances generated by reaction between the free coenzyme and a color developingsolution is detected through the spectrophotometry, thereby indirectly calculating the content of the L-carnitine in a sample. An experimental result shows that the addition amount of an acetylcoenzyme solution is 80 [mu] L, the addition amount of a carnitine acetyltransferase solution is 100 [mu] L, the reaction time is 5 minutes, and accurate, economic and rapid detection requirements can be met.

Description

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Claims

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Application Information

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Owner ZHENJIANG XIANCHUANG BIOTECH CO LTD
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