Sarcopenia animal model establishing method

A technology of animal model and establishment method, which is applied in the medical field, can solve the problems that are not conducive to the early research objects of the diagnosis and treatment of sarcopenia syndrome, lack of indirect animal experimental models, etc., and achieve good reproducible results

Pending Publication Date: 2020-08-14
SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves upon existing methods by making it easier than previously possible or more cost-effective while still providing stable results that may be used to develop new drugs targeting specific diseases like sarcopenia (satellite cell death).

Problems solved by technology

There has been no clear relationship between different symptoms like sarcoplasmatic degenerative changes or cognitive impairment caused by advanced illnesses including Alzheimer’s Disease, Parkinsonism, multiple neurologies, autistic spectrum disorder, Autism Spectrum Disorder, etc., indicating an important role played during these complex physiopathologies. Current methods involve subjectively assessing body weight and musculoskeleton weakening through questionnaires alone without considering any underlying factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention provides a method for establishing a sarcopenia animal model, and the experimental process and principle of the present invention are illustrated below in conjunction with specific examples:

[0025] a laboratory animal

[0026] 72 4-week-old C57BL / 6 male mice were purchased and raised in separate cages. They were given normal mouse feed, free access to food and water, light for 12 hours / day, temperature at 20-25°C, and humidity at 40-60%.

[0027] Two experimental treatments

[0028] From the age of 1 month, C57BL / 6 male mice were divided into pairs to measure body weight, body composition and muscle function (muscle mass, grip strength, endurance and muscle fiber cross-sectional area indicators) once a month. Then 3 mice were randomly selected, anesthetized by subperitoneal injection of 7% chloral hydrate solution (purchased from Beyuntian Biotechnology Research Institute), and the skeletal muscles of the lower limbs (anterior tibialis muscle

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Abstract

The invention discloses a sarcopenia animal model establishing method. The method comprises the following steps of purchasing 4-week-old male mice, and feeding the 4-week-old male mice in different cages; measuring the body weight, body composition and muscle function of male mice once in pairs every month from the age of 1 month, randomly selecting three mice, performing intraperitoneal injectionof 7% chloral hydrate solution for anesthesia, taking out skeletal muscles of lower limbs, weighing, preparing a frozen specimen, transferring the frozen specimen into a refrigerator at the temperature of -80 DEG C, and storing for later use; fixedly observing and recording mice muscle function measurement results every month, and regarding that modeling is successful when a month mean value of the mice muscle function measurement results is reduced. The experimental difficulty, experimental conditions, economical efficiency and the like of the method are greatly improved compared with thoseof an existing model, an animal model which is stable, good in repeatability and closer to clinic in mechanism can be provided, and a good animal model sample is provided for studying the molecular mechanism, the pathophysiological characteristics and drug research and development of the sarcopenia.

Description

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Claims

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Application Information

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Owner SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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