Composition comprising elecampane extract for alleviating premenstrual syndrome symptom

[0067]Aucklandiae radix extracts were prepared in the same manner as in Example 1, except that the concentration of ethanol during extraction was 50% (Example 2) or 70% (Example 3).

Experimental Example 1: Measurement of Efficacy of Inhibition of Prolactin Secretion

[0069]The efficacy of inhibition of prolactin secretion by the Aucklandiae radix extracts obtained in Examples and Comparative Examples (Comparative Examples 1 to 3) were measured using pituitary cells.

[0070]Rat pituitary cells GH3 were purchased from the American Type Culture Collection (ATCC), DMEM culture medium, trypsin, and fetal bovine serum (FBS, Gibco™) were purchased from Invitrogen Corporation, and a prolactin ELISA kit for measurement was purchased from Mol-innovation.

[0071]GH3 pituitary cells were seeded at a concentration of 4×104 cells / well into a 24-well plate and incubated for 24 hours. In order to evaluate the efficacy of inhibition of prolactin secretion, the cells were treated with 25 and 50 μg / mL of the Aucklandiae radix extracts of Examples 1 to 3 and Comparative Examples 1 to 2 and then incubated for 48 hours, and prolactin secretion was assayed by application to a standard cur

of Ameliorating Premenstrual Syndrome in Hyperprolactinemia-Induced Animal Model

[0074]Metoclopramide (MCP) is a substance used as a vomiting inhibitor and is known as a drug that increases the secretion of prolactin by inhibiting the secretion of dopamine, and thus inhibits the secretion of progesterone, luteinizing hormone, and follicle-stimulating hormone (Wang et al., 2014). Accordingly, in the present invention, an animal model with premenstrual syndrome induced by hormone secretion disorders was established by administering 20 mg / kg of metoclopramide into the abdominal cavity of female mice every two days for a total of 20 days and then inducing hyperprolactinemia.

[0075]ICR mice (female, 12 weeks old) were obtained from Raon Bio Co., Ltd. and were acclimated for 1 week while normal solid feed and water were freely supplied thereto. The environmental conditions of the rearing room were set to a temperature of 23±3° C., a relative humidity of 50±10%, an illumination time of 12 hou