2 results about "Bovine serum albumin" patented technology

The invention discloses a method for quickly detecting aflatoxin B1 by PbS quantum dot. The method is characterized by comprising the steps of: adding Pb(NO3)2 solution into mercaptoacetic acid and Na2S solution to prepare PbS quantum dot; preparing 10mug/mL QD-Ab solution by coupling of PbS quantum dot and aflatoxin B1 monoclonal antibody; adding aflatoxin B1-bovine serum albumin conjugate in an elisa plate to coat the elisa plate, and simultaneously adding AFB1 solution and QD-Ab solution, after incubation digestion, adding Hg2Cl solution, getting constant volume by adding acetic acid buffer solution, then preparing the to-be-detected solution; deoxidizing the to-be-detected solution, adding the deoxidized to-be-detected solution into a pretreated glassy carbon electrode for detection, and calculating the content of the aflatoxin B1 according to a standard curve relation between the dissolving peak size of Pb<2+> and the concentration of aflatoxin B1 solution. The method has the advantages of high sensitivity, strong selectivity, excellent stability, high accuracy and quick detection speed.

The invention discloses an electrochemical sensor electrode for rapidly and simultaneously detecting lead ions and cadmium ions as well as a preparation method and application of the electrochemical sensor electrode, and belongs to the technical field of electrochemical sensors. According to the invention, a bovine serum albumin-aminated graphene oxide-glutaraldehyde cross-linking material is synthesized in situ on the surface of a screen-printed carbon electrode, and a flower-like bismuth tungstate material is loaded on the surface of the electrode by a dispensing method, so that an electrode sensitive interface of the sensor is constructed; and simultaneous square wave anodic stripping voltammetry detection of trace lead and cadmium ions is realized. Compared with the prior art, the method has the advantages of high sensitivity, accurate result, simplicity in operation, easiness in carrying, recyclability and the like, and the heavy metals lead and cadmium in the water body can be rapidly and quantitatively analyzed and detected at the same time without pretreatment.