The invention discloses a preparation method of an anglerfish bone collagen peptide and active calcium health-care oral liquid. The anglerfish bone is cleaned, crushed and sieved, then added in NaOH solution, soaked to remove miscellaneous proteins; after repeated washing and draining, adding acrylic acid Alcohol solution, soaked to remove fat, and dried to obtain defatted fish bone powder; Enzymolysis was carried out by endopeptidase, and combined with ultrasonic-microwave assisted enzymolysis; the obtained enzymolysis solution was heated, inactivated, centrifuged, and the supernatant was concentrated. The anglerfish bone collagen peptide concentrate is obtained; the remaining fish bone powder is dried, added with lactic acid, activated, adjusted for pH, filtered, heated in a water bath, and concentrated to obtain the active calcium lactate concentrate. The preparation method of the invention is simple, scientific and reasonable, utilizes raw materials to the greatest extent, and is environmentally friendly and economical.