An anti-interference determination method for an
enzyme electrode biosensor comprises the following steps: constant a and Michaelis constant b are calculated by firstly measuring corresponding currentvalue X of a to-be-tested sample, then measuring corresponding current value Y of the to-be-tested sample and equivalent standard sample and finally measuring corresponding current value Z of the to-be-tested sample and half of the standard sample. Concentration of the to-be-tested sample after compensation is calculated finally, influence of interference factors is eliminated in the determination method, and a determination result obtained with the determination method is more accurate and more reliable under the condition of presence of an interference source.