The invention relates to the fields of biomedical engineering, computer technology and communication, and specifically discloses an accurate positioning method of a rehabilitation FES signal for stroke patients. The method comprises the steps of wearing a dynamic exoskeleton, defining sensor parameters, performing healthy joint movement, recording exercise data of a healthy joint and the myoelectric signal data of adjacent limbs, and calculating the corresponding motion data of an affected joint and the action time and action point of the FES. The accurate positioning method determines a functional electrical stimulation site and a timing of the affected joint through the healthy joint motion of the patients, instead of determining by the experience of a rehabilitation engineer, by using the principle of time-lapse mirroring, each patient can find electrical stimulation time and action positions suitable for own physical conditions, the FES stimulation on the patients makes the actionof the patient affected joint closer to the action of normal limbs, which can better improve the activation of the brain mirror neurons and promote the rehabilitation of the patients, and the method can help the stroke patients to correct gait during the rehabilitation process to a certain extent.