The invention discloses a preparation method of a hand-washing-free disinfection preparation, and relates to the technical field of sanitary disinfection supplies. The hand-washing-free disinfection preparation is prepared by taking chemical disinfection components and plant disinfection components as effective components of the disinfection preparation and adding a gel matrix. The prepared hand-washing-free disinfection preparation is low in ethanol content and low in concentration, and mainly takes water as a diluent, so that the hand-washing-free disinfection preparation is suitable for crowds being allergic to alcohol, and the skin problem caused by high-concentration alcohol is solved. The prepared hand-washing-free disinfection preparation has the advantages of obvious disinfection effect and high killing rate, can quickly kill pathogenic bacteria carried by hands in a short time, and is very convenient to use.