2 results about "Glycopeptide" patented technology

The invention belongs to the field of protein analysis, and relates to a new method for enrichment of a glycosylated peptide through a magnetic nanometer material modified by aminoxy, in particular to a method for solid-phase enrichment and mass spectrographic analysis of a glycosylated peptide fragment. The method comprises the following steps: firstly, using sodium periodate NaIO4 to oxidize a hydroxyl group on a glycopeptide to change the hydroxyl group to an aldehyde group; secondly, placing the magnetic nanometer material Fe3O4@ONH2 modified by aminoxy to an oxidized glycopeptide solution, and fixing the glycopeptide to the magnetic nanometer material through reaction of an aminoxy group and the aldehyde group obtained after a sugar chain in the glycopeptides is oxidized; thirdly, cleaning to remove non-glycopeptide which does not react with the nanometer material; finally, dissociating the captured glycopeptide from the material by use of sugar chain enzyme, and realizing mass spectrographic analysis of the glycosylated peptide. The method is simple in step, convenient to operate, high in speed and efficient, and can realize highly-sensitive and highly-selective mass spectrometric analysis of the glycosylated peptide.

An assay system designed to detect a protein biomarker in urine that is diagnostic for interstitial cystitis (IC). The presence of a 9 amino acid glycopeptide, antiproliferative factor (APF), in urine is unique to patients with IC. Urine samples from patients who exhibit symptoms consistent with IC are added to the assay system. Binding of APF to the cytoskeletal associated protein 4 (CKAP4) is positive for the presence of APF in urine and diagnostic for IC. The diagnostic system is a significant and surprising advance in diagnosis of IC and has commercial applications relevant to women and men who suffer from symptoms consistent with IC.