2 results about "Primary cell" patented technology

The invention relates to the technical field of targeted editing of a buffalo 18S rDNA gene, in particular to a method for targeted editing of the buffalo 18S rDNA gene by virtue of an adenovirus system. According to the method provided by the invention, an autonomously constructed 18S gene editing vector is used for editing the buffalo 18S rDNA gene; a constructed adenovirus vector consists of Cas9 protein and sgRNA; and then, the vector is subjected to adenovirus packaging. The buffalo 18S rDNA gene is edited on the basis of the accurate and efficient performances of a Cas9 editing gene, a strong bearing capacity of adenovirus on exogenous genes as well as a strong infection capacity of the adenovirus on primary cells, so that a bottleneck that the buffalo primary cells are difficult for transfection; and the packaged adenovirus can efficiently infect buffalo primary mammary epithelial cells and can efficiently edit the buffalo 18S rDNA gene.

Embodiments of the present disclosure relate to handover. In response to receiving a request for handover of the second device from the third device to the first device, the first device sends to the third device an indication for a radio bearer of the second device to change from a separate bearer terminated in the fourth device to a primary cell group bearer terminated in the first device, and the second equipment is dual-connected with the third equipment and the fourth equipment. The first device receives, from a third device, a transmission status of data associated with the radio bearer.