1 results about "Primary screening" patented technology

The invention discloses a high ribonucleic acid saccharomyces cerevisiae strain and application thereof, and belongs to the technical field of bioengineering. According to the invention, the normal pressure and room temperature plasma mutagenesis technology (ARTP) is used for mutagenizing an original strain (9.20%), and the KS culture medium is used for primarily screening the mutagenic strain, so that the screening efficiency is greatly improved. The JNB-G03H8 with excellent characters is obtained by re-screening in the plurality of obtained high-yield ribonucleic acid yeasts. The RNA content of the strain is high, after shake-flask culture in a normal YPD culture medium, the RNA content (g/DCW) of the strain can reach 12.89%, the RNA content of the strain can reach 15.80% at most under the condition of an optimized culture medium through a response surface optimization experiment, compared with 9.20% of an original strain G03, the RNA content of the strain is improved by 70.76%, and the strain is a non-transgenic strain, non-toxic, harmless, stable in heredity and safe and reliable when applied to the field of food.