3 results about "Titer" patented technology

The invention provides a preparation method of monoclonal antibodies for IC (nfectious coryza) of chickens. According to the technical scheme, hybridoma cell strains secreting the monoclonal antibodies against avibacterium paragallinarum are prepared from B type strains, the secreted antibodies are only targeted at the B type strains and do not have reactions with A type and C type avibacterium paragallinarum, and accordingly, the antibodies are only targeted at B type serotype diseases. Besides, an ELISA (enzyme-linked immunosorbent assay) method and an immune colloidal gold method established according to the characteristics of the monoclonal antibodies have good specificity and sensitivity and can be used for clinical detection. Besides, the monoclonal antibodies prepared with the method are high in titer, better in specificity and especially applicable to further preparation of B type avibacterium paragallinarum antigen diagnostic kits or B type avibacterium paragallinarum antigen diagnostic colloidal gold test paper. Prominent technical effects are realized through innovative technical improvements, and the preparation method is lower in cost and easy to implement and has prominent popularization prospect.

The invention discloses an enterovirus 71 double antibody sandwiched ELISA kit. The kit comprises an enzyme label plate coated with an anti-VP1 polyclonal antibody, an enzyme-labeled anti-VP1 polyclonal antibody and a VP1 antigen. The invention also discloses a preparation method and a detection method of the kit. The kit is prepared from the anti-VP1 polyclonal antibody and the enzyme-labeled anti-VP1 polyclonal antibody. The preparation method has simple processes. The kit realizes fast detection and standardization. Through use of high bonding titer of the anti-VP1 polyclonal antibody to various EV 71 genotypes, the detection result is more accurate.

The invention relates to a preparation method of thrombin, which comprises the following steps: filtering to remove impurities in porcine plasma; adding a virus inactivation reagent to perform virus inactivation treatment; adding an adsorbent to adsorb prothrombin; washing and immersing the prothrombin-adsorbed adsorbent, and dialyzing; activating the prothrombin in the dialyzed solution; regulating the pH value of the solution to 5.2-5.6, standing, centrifugating, regulating the pH value of the supernatant to 6.0-6.5, and standing; passing through a membrane adsorber, and eluting to obtain an eluate; filtering through an ultrafiltration membrane; filtering through a filter membrane for sterilization, and filtering through a filter with the pore size of 20nm to remove viruses; and preparing the preparation. On the basis of further stabilizing the titer of the thrombin product, the method effectively reduces the impurities in the thrombin product and the concentration of the impurity proteins, and implements virus removal after sufficient inactivation.