On-line rapid detection method of stevioside

A steviol glycoside and solution technology, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve practical application difficulties such as breeding and cultivation production monitoring at any time, thin-layer chromatography has many chemical reagents, The instrument is inconvenient to carry and other problems, and achieves the effect of high sensitivity, low detection cost and simple operation

Inactive Publication Date: 2011-11-23
GUILIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

[0006] Disadvantages of various detection methods: Thin-layer chromatography uses more chemical reagents, which is suitable for the purification of small samples; high-pressure liquid chromatography and capillary electrophoresis instruments are relatively expensive, and professional and technical personnel are required at the same time, generally steviol glycosides The processing plant is not easy to realize, and the mobile phase and chromatographic column of special chromatographic purity are relatively expensive, and the instruments such as high-pressure liquid chromatography and capillary electrophoresis are not convenient to carry, and are practically used in the breeding and cultivation production of agricultural stevia, such as monitoring at any time tougher

Method used

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Example Embodiment

[0012] Examples:

[0013] Use 0.1mol / L H 2 SO 4 Prepared as a solvent 5.0×10 -4 mol / L cerium ammonium sulfate (Ce(IV)), using double distilled water as solvent to prepare 1.0×10 -2 mol / L Na 2 SO 3 For the solution, use twice-distilled water as a solvent to prepare a standard solution of 10 mg / mL steviol glycoside and a sample steviol glycoside solution. The active pump separates the above-mentioned carrier current Na 2 SO 3 The solution and the steviol glycoside standard solution were mixed through the corresponding pipes at a flow rate of 45r / min for three-way mixing, and then input into the flow injection chemiluminescence analyzer. After the baseline is stable, the auxiliary pump passes the cerium ammonium sulfate solution through the sixteen-way injection valve at a flow rate of 65r / min. The injection time is 10 seconds. The liquids are mixed and flowed into the flow cell to detect the chemiluminescence intensity, record the data, and make a standard Curve equation;

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Abstract

The invention discloses an on-line rapid detection method of stevioside, comprising the following steps of: preparing an ammonium ceric sulfate solution (Ce(IV)), a Na2SO3 solution, a stevioside standard solution and a sample stevioside solution; respectively mixing the above Na2SO3 solution and the stevioside standard solution in a three-way valve by a driving pump via a corresponding pipeline at the flow speed of 30-60 r/min, and inputting the mixed solution into a chemical luminescence analytical device of flow injection; letting the ammonium ceric sulfate solution flow through a sixteen-way valve at the flow speed of 50-80 r/min by an auxiliary pump for the injection detection time of 5-15 seconds after a baseline becomes stable, letting each solution mix and flow into a flow-through cell to detect the chemiluminescence intensity of the mixed solution, recording data, and making a standard curvilinear equation; repeating the above operation, testing the chemiluminescence intensity of the sample stevioside, substituting into the standard curvilinear equation, and calculating the content of the sample stevioside. The detection method provided by the invention is simple, requires low detection cost, can be used for on-line routine detection, and is applicable to industrial or agricultural production.

Description

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Claims

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Application Information

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Owner GUILIN UNIVERSITY OF TECHNOLOGY
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