Marine fungus penicillium herquei, its extract and application

[0027] Example 1: Isolation and identification of marine fungus Penicillium herquei FS83

[0028] The marine fungus Penicillium herquei (Penicillium herquei) FS83 was isolated and purified from the sea mud at a depth of 1675m in the South China Sea (106°30.295′E, 10°0.941′N).

[0029] The method of separation and purification is as follows: dilute the sea mud sample to 10% with sterile water containing 30% coarse sea salt, shake at 27°C for 20 minutes, and apply 0.1 mL uniformly on the modified PDA solid medium, in a thermostat at 26-28°C After the colony grows, the colony is picked and streaked to purify it. The improved PDA solid medium is to add KH per liter of PDA medium 2 PO 4 3g, MgSO 4 0.75g, vitamin B 1 10mg, 30g coarse sea salt, 20g agar.

[0030] Through morphological research and according to "Fungus Identification Manual" (Wei Jingchao, 1979), the strain was identified as Penicillium herquei (Penicillium herquei), named Penicillium herquei FS83, on November 24, 2011 Deposi

[0033] Example 2: Preparation of fermented extract of marine fungus Penicillium herquei FS83

[0034] The marine fungus Penicillium herquei (Penicillium herquei) FS83 was inoculated into a modified PDA liquid medium and cultured at 120 r / min at 26°C for 7 days to obtain a liquid culture of the marine fungus Penicillium herquei FS83. The liquid culture of the marine fungus Penicillium herquei FS83 is filtered to separate the mycelium from the fermentation broth. The mycelium is cold soaked with petroleum ether or acetone or absolute ethanol or 95% ethanol aqueous solution. This example Cold soaked in absolute ethanol for 48h, and then the cold soaked liquid was concentrated under reduced pressure to obtain a green paste-like extract, which is the fermented extract of marine fungus Penicillium herquei FS83.

[0035] The modified PDA medium is obtained by the following method: 200g potatoes are boiled in 500mL distilled water for 20 minutes, filtered to obtain the juice, and then 20g g

[0036] Example 3: Antibacterial activity of marine fungus Penicillium herquei (Penicillium herquei) FS83 fermentation extract

[0037] One,

[0038] The filter paper method was used to determine the antibacterial activity of the marine fungus Penicillium herquei FS83 fermentation extract against Bacillus subtilis, Staphyloccocus aureus and Pseudomonas aeruginosa.

[0039] The marine fungus Penicillium herquei FS83 fermentation extract obtained in Example 2 was dissolved in DMSO and diluted to a concentration of 50 mg / mL.

[0040] Dilute the above-mentioned bacterial solution to be tested obtained by liquid fermentation with physiological saline to 10 6 The concentration of CFU / mL, with a concentration of 10 6 Spread 200μL of CFU / mL bacterial solution evenly on a plate containing LB medium, paste a filter paper sheet with a diameter of 4mm, and drop the marine fungus Penicillium herquei with a concentration of 50mg / mL on the filter paper sheet. 10μL of FS83 fermentation extract di