Preparation method of in vivo glycation level-improving dementia animal model

An animal model, a horizontal technology, applied in the direction of medical preparations, drug combinations, pharmaceutical formulations containing active ingredients, etc., can solve problems such as renal tissue toxicity damage, damage, etc., to achieve the short time required for modeling and low drug toxicity. , the effect of improving the level of glycation

Inactive Publication Date: 2013-01-30
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Application Information

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Benefits of technology

This new method described in this patents allows researchers studying an organism's metabolic processes without increasing their own risk or harmful side-effects on them during experiments that were conducted previously.

Problems solved by technology

Technologies aimed at developing new ways to improve understanding how biomolecusis occurs during inflammations caused by various causes like autoimmunity, oxidant stress, gene mutants, cancer chemotherapy agents, etc., while avoiding these negative consequences associated with current methods. Previous studies showed promising results from studying different types of enzymes involved in carboxypolysis processes, including Gα(C)-binding adhesion moleculae group members and their interactions. Additionally, previous experiments conducted over several decades ago revealed potential connections among many genome structures, suggesting that there could exist multiple forms of garniscibility within each cell type. Overall, the technical problem addressed by this patents relates to improving the effectiveness of existing approaches based on modifying specific parts of the subject being studied without causing unwanted health issues.

Method used

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  • Preparation method of in vivo glycation level-improving dementia animal model
  • Preparation method of in vivo glycation level-improving dementia animal model
  • Preparation method of in vivo glycation level-improving dementia animal model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Male C57BL / 6J mice (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.), with a body weight of about 20 grams, were adaptively fed for one week, and the animals were randomly divided into 5 groups, normal saline control group and four different concentration groups. D-ribose (purchased from Amresco, USA) was administered to the model group, 12 rats in each group. The concentration of intraperitoneal injection of D-ribose in the administration group was respectively: 400mg / kg / day, 800mg / kg / day, 1600mg / kg / day, 3200mg / kg / day (prepared with normal saline), continuous injection for 10 days, and intraperitoneal injection in the control group. equal volume of saline.

[0032] Model test results:

[0033] 1. Increased concentration of glycosylated serum protein

[0034] After the administration, blood was collected from the mice in each group, and the serum was collected after static centrifugation to measure the concentration of glyc

Embodiment 2

[0040] C57BL / 6J male mice, weighing about 20 grams, were randomly divided into 3 groups after one week of adaptive feeding, normal saline control group and two different concentrations of D-ribose administration modeling groups, 15 in each group. The concentration of D-ribose injected intraperitoneally in the administration group was respectively: 200 mg / kg / day and 2000 mg / kg / day (prepared in normal saline), for continuous injection for 30 days, and the same volume of normal saline was injected intraperitoneally in the control group.

[0041] Model test results:

[0042] 1. Increased concentration of glycosylated serum protein

[0043] After the administration, blood was collected from the mice in each group, and the serum was collected after static centrifugation to measure the concentration of glycated serum protein in the serum of the mice in each group (the detection kit for the concentration of glycated serum protein was from Nanjing Jiancheng Bioengineering Resear

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Abstract

The invention relates to a preparation method of an in vivo glycation level-improving dementia animal model. By dosing animals with D-ribose, the invention provides an in vivo glycation level-improving dementia animal model and its preparation method. The animal model provided in the invention has the characteristics of normal blood sugar level, enhanced glycation level, and damaged spatial learning and memory ability. At the same time, the method disclosed in the invention has the advantages of simplicity, practicability, easy control, stability and good reproducibility, low model making cost as well as short model making cycle. The model making method provided in the invention can be used for qualitative and quantitative analysis, pathomorphological contrastive analysis, and also can be used for statistical treatment and analysis. Also, the method can be used for studying in vivo glycation level enhancement-caused related diseases and the pathogenesis of neurodegenerative diseases, as well as studying and evaluating related disease treatment methods.

Description

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Claims

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Application Information

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Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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