SNP (single nucleotide polymorphism) marker related to Erhualian sow litter traits and detection method and application thereof

[0028] 1. Source of experimental animals

[0029] Jiaoxi Erhualian pig professional cooperative in Changzhou, Jiangsu.

[0030] Calculate the breeding value of 177 Erhualian sows, and the calculation model is Y (total litter size) = parity (parity) + farm (field) + year (annual) + season (season) + age (age of sow farrowing )+sire (with boars)+permanent effect+additive effect (permanent effect of sows)+e (residual),

[0031] These include fixed effects - parity, farm / year / season sow farrowing, covariates - sow age at farrowing, random

[0032] Effects - mated boars, permanent effects - sows, individual additive genetic values. Select 18 individuals whose breeding value ranks first or last and whose litter record is more than 3 litters.

[0033] 2. Genomic DNA extraction

[0034] Ear tissue samples from 36 sows were collected, placed in centrifuge tubes filled with 70% alcohol, and stored in a -20°C refrigerator for later use.

[0035] Using the traditional phenol / chlor

[0059] This example is the verification of the SNP site g.117011153A / G obtained in Example 1 in the Erhualian sow population.

[0060] 1. Extraction of Genomic DNA from Erhualian sows

[0061] Ear tissue samples from 131 purebred Erhualian sows with accurate records of total litter size were collected, placed in centrifuge tubes filled with 70% alcohol, and stored in a -20°C refrigerator for later use. Genomic DNA from ear tissue was extracted by the above method, and after quality and concentration detection, the concentration was diluted to 30 ng / μL and stored at -20°C for future use.

[0062] 2. PCR amplification and sequencing of the target fragment

[0063] Using the extracted DNA as a template, carry out PCR amplification according to the designed primers: take 2.5 μL of DNA template, 1.25 μL of primers shown in SEQ ID NO: 2 and SEQ ID NO: 3, 25 μL of PCR Mix reagent, double 20 μL of distilled water; set up the PCR amplification system: pre-denaturation at 96°C for 2