Characteristic map detection method of powerful gastrodia elata-eucommia ulmoides capsules

A technology of Tianma, Eucommia, and characteristic map, which can be used in measuring devices, instruments, scientific instruments, etc., can solve problems such as deficiencies, and achieve the effect of accurate methods.

Active Publication Date: 2016-03-16
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the characteristic map of Qiangli Tianma Duzhong Capsules. Therefore, for the iden

Method used

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  • Characteristic map detection method of powerful gastrodia elata-eucommia ulmoides capsules
  • Characteristic map detection method of powerful gastrodia elata-eucommia ulmoides capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1 Instruments and materials:

[0017] UltiMate3000 high performance liquid chromatography (including system controller, infusion pump, degassing components, low-pressure gradient components, autosampler, column thermostat, temperature-controlled sample chamber, UV-DAD detector, Chromeleon chromatography data workstation), AE240 electronic balance (Mettler-Toledo Instruments Shanghai Co., Ltd.), CQ-250A-ST ultrasonic cleaner (Shanghai Yuejin Medical Optical Instrument Factory), ultra-pure water machine (Sichuan Water Technology Development Co., Ltd.).

[0018] 10 batches of Qiangli Tianma Eucommia Capsules (11254011, 11254018, 12254001, 12254002, 13254001, 13254003, 14254001, 14254002, 14254004, 14254006, code S1~S10) were provided by Guizhou Hongyu Pharmaceutical Co., Ltd.; , Gastrodin (110807-201306) was purchased from China National Institutes for Food and Drug Control; procuridine, 7-hydroxycoumarin, protocatechuic acid, ascarin, and gallic acid were obtained from Qiang

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Abstract

The present invention discloses a characteristic map detection method of powerful gastrodia elata-eucommia ulmoides capsules. According to the characteristic map detection method, a test sample solution is prepared, wherein the test sample solution contains the site A of an alkaline condition ethyl acetate extraction sample and the site B of an acidic condition ethyl acetate extraction sample; a reference substance solution is prepared; the test sample solution and the reference substance solution are respectively subjected to chromatographic condition determination; the characteristic maps respectively generated from the data of the sites A and B of the test sample solution are subjected to a precision test, a stability test and a repeatability test; the characteristic map of powerful gastrodia elata-eucommia ulmoides capsules is established, and is subjected to similarity evaluation; and finally the attribution of the chromatographic peak is determined so as to identify the components of the powerful gastrodia elata-eucommia ulmoides capsules. The method of the present invention is accurate and reliable, and can be used for the identification and the quality control of the powerful gastrodia elata-eucommia ulmoides capsules.

Description

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Claims

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Application Information

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Owner GUIZHOU MEDICAL UNIV
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