Alkaloid compound with antibacterial activity in tobaccos as well as preparation method and use of alkaloid compound

[0027] Preparation of alkaloid compound C 15 h 13 NO 3 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:

[0028] 1. Extraction of extract: take tobacco leaves and pulverize them, use high-concentration methanol (w%: 95%) or high-concentration ethanol (w%: 95%) or high-concentration acetone (w%: 70%) as extraction solvent, extraction solvent: Tobacco (weight ratio)=3:5, soaked for 54h, extracted 4 times, combined extracts, filtered and concentrated into extract.

[0029]2. Silica gel column chromatography: dissolve the extract with 2.5 times the weight ratio of pure methanol or pure ethanol or pure acetone, mix the sample with 1.2 times the weight ratio of 80-100 mesh silica gel, and use 3 times the weight ratio of 250 mesh silica gel Dry packing for silica gel column chromatography; chloroform in volume ratios (1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1 and 1:2) - Gradient

[0034] The tobacco samples were from Yuxi, Yunnan, and the variety was Yuxi K326. Sampling 2.0 kg of tobacco was pulverized and extracted 5 times with 95% methanol for 24 hours each time. The extracts were combined, filtered, and concentrated under reduced pressure to form an extract to obtain 105 g of extract. After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 120g of 100-mesh silica gel, pack 0.6kg of 160-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8: The chloroform-acetone elution part of 2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 52% methanol as mobile phase, ZorbaxSB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, flow rate was 20ml / min, the d

[0036] The tobacco sample comes from Dali, Yunnan, and the variety is Yunyan 200. The tobacco sample is 3.5kg and chopped, and extracted 4 times with 95% ethanol, each extraction is 48h, the extracts are combined, filtered, and concentrated under reduced pressure to obtain an extract. 250g. The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 250 g of 80 mesh thick silica gel, and 1.2 kg of 200 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8: The chloroform-acetone elution part of 2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 52% methanol as mobile phase, ZorbaxSB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, flow rate was 20ml / min, the detection wavelength of the u