Whitening composition containing folium artemisiae argyi stem cell extract and application thereof
A composition and stem cell technology, applied in the directions of medical preparations containing active ingredients, cosmetic preparations, preparations for skin care, etc., can solve problems such as skin irritation and skin side effects, and achieve improved whitening effect and good whitening The effect of inhibiting bacterial growth
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[0043] Example 1
[0044] Example 1 provides a method for preparing a stem cell extract of mugwort leaf, which includes the following steps:
[0045] ①Take the root tissue of the aseptic wormwood seedling containing the quiescent center, remove the root cap, and cut 1mm from the incision as an explant;
[0046] ②Put the explants into an induction medium with pH 6.0, and conduct dark culture at 25°C for 30 days. The quiescent center stem cells are separated from other root tissue cells to obtain quiescent center stem cells; the induction medium contains NAA, MS solid medium of BA and hydrolyzed casein; wherein the concentration of NAA is 0.5 mg / L, the concentration of BA is 2.0 mg / L, and the concentration of hydrolyzed casein is 1.0 mg / L;
[0047] ③Transfer the quiescent stem cells to a growth medium with pH 6.0, culture at 25°C on a shaker for 10 days and filter with a 100 mesh stainless steel screen to remove cell clusters and residues to obtain a single cell suspension; shake cul
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[0050] Example 2
[0051] The present Example 2 provides a whitening composition consisting of the following components by weight: 0.2 parts of mugwort stem cell extract, 0.8 parts of ginseng extract, 20 parts of lily extract, and 20 parts of chrysanthemum extract.
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[0052] Example 3
[0053] This example 3 provides a whitening composition, which is composed of the following components by weight: 0.3 parts of mugwort stem cell extract, 1 part of ginseng extract, 25 parts of lily extract, and 25 parts of chrysanthemum extract.
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