Method for reducing background coated by using enzyme-linked immunosorbent assay (ELISA)

[0020] Embodiment 1: a kind of method for reducing the background after ELISA coating, it comprises the following steps:

[0021] S1. Preparation of coating solution: take 0.05M carbonate solution, add macromolecular substance surfactant S6 with a mass percentage concentration of 1%, and mix evenly, which is the coating solution; the carbonate solution and macromolecular substance The volume ratio of surfactant S6 is 10000:5;

[0022] S2. Coating: Take the coating protein antigen Mycobacterium tuberculosis 38KD protein, add the coating solution prepared in step S1, leave it overnight at 4°C for 12 hours, and discard the liquid;

[0023] S3. Blocking: adding 1% bovine serum albumin in PBS buffer to the protein antigen coated in step S2 for blocking, and standing at 37° C. for 1.5 h.

[0024] Embodiment 2: a kind of method that reduces the background of ELISA coating, it comprises the following steps:

[0025] S1. Preparation of coating solution: take 0.05M carbonate solution, add macromolecular substance surfactant S6 with a mass percentage concentration of 1%, and mix evenly, which is the coating solution; the carbonate solution and macromolecular substance The volume ratio of surfactant S6 is 10000:20;

[0026] S2. Coating: Take the coating protein antigen Mycobacterium tuberculosis 38KD protein or Mycobacterium tuberculosis secreted acid phosphatase, add the coating solution prepared in step S1, leave it overnight at 8°C for 16 hours, and discard the liquid;

[0027] S3. Blocking: adding 1% bovine serum albumin in PBS buffer to the protein antigen coated in step S2 for blocking, and standing at 37° C. for 2.5 hours.

[0028] Embodiment 3: a kind of method that reduces the background of ELISA coating, it comprises the following steps:

[0029] S1. Preparation of coating solution: take 0.05M carbonate solution, add macromolecular substance surfactant S6 with a mass percentage concentration of 1%, and mix evenly, which is the coating solution; the carbonate solution and macromolecular substance The volume ratio of surfactant S6 is 1000:1;

[0030] S2. Coating: Take the coating protein antigen Mycobacterium tuberculosis 38KD protein or Mycobacterium tuberculosis secreted acid phosphatase, add the coating solution prepared in step S1, leave it overnight at 6°C for 14 hours, and discard the liquid;

[0031] S3. Blocking: adding 1% bovine serum albumin in PBS buffer to the protein antigen coated in step S2 for blocking, and standing at 37° C. for 2 hours.

[0032] The beneficial effect of the present invention is illustrated by experiment below:

[0033] 1. Buffer blank coating, background level de