Composition for treating lung cancer

[0060] The extraction of embodiment 1 crocin

[0061] Soak the dried stigma of saffron in 400ml of 75 / 25 (V / V) methanol-water solution for 30 minutes, ultrasonically extract for 30 minutes, pour out the leaching solution, repeat the extraction three times, combine the saffron leaching solution obtained, vacuum filter, 50-60 ℃ condition The methanol was distilled off in a rotary evaporator.

[0062] Soak the D101 resin in 95% ethanol for 24h, wet the column with ethanol, and put the packing in the glass column, packing 50cm. Dissolve the obtained saffron crude extract in water, add it to the preparation column, and carry out gradient elution with ethanol-water solution: at 0-3h, the volume ratio of ethanol to water is 20:80; at 3-7h, ethanol The volume ratio of ethanol to water is 40:60, 7-9h, the volume ratio of ethanol to water is 60:40, 9-11h, the volume ratio of ethanol to water is 80:20, 11-14h, the volume ratio of ethanol to water The volume ratio is 95:5. Collect eac

[0063] Embodiment 2 Preparation of Erlotinib and Albumin Nanoparticle Composition Loaded with Crocin

[0064] (1) Preparation of saffron-loaded albumin nanoparticles;

[0065] 1) mixing the crocin drug in the organic phase, wherein the crocin drug is 10% of the organic phase;

[0066] 2) Dissolving albumin completely in deionized water, wherein the albumin is 1% of the aqueous medium;

[0067] 3) Shearing at a rotational speed of 5000 rpm for 5 minutes, slowly injecting the organic phase into the water phase while dispersing to obtain colostrum;

[0068] 4) Under the condition of 40000psi, the colostrum was subjected to high-pressure homogenization and repeated 5 times to obtain a homogeneously dispersed saffron-loaded albumin nanoparticle suspension;

[0069] 5) Rotating the obtained saffron-loaded albumin nanoparticle suspension at 40° C. for 60 minutes in vacuum to remove the organic phase;

[0070] 6) The saffron-loaded albumin nanoparticle suspension was passed through a

[0072] Example 3 Preparation of Erlotinib and Albumin Nanoparticle Composition Loaded with Crocin

[0073] (1) Preparation of saffron-loaded albumin nanoparticles;

[0074] 1) mixing the crocin medicine in the organic phase, wherein the crocin medicine is 80% of the organic phase;

[0075] 2) Dissolving albumin completely in deionized water, wherein the albumin is 10% of the aqueous medium;

[0076] 3) Shear at a rotational speed of 40,000 rpm for 5 minutes, and slowly inject the organic phase into the water phase while dispersing to obtain colostrum;

[0077] 4) Under the condition of 6000psi, the colostrum was subjected to high-pressure homogenization and repeated 30 times to obtain a homogeneously dispersed suspension of albumin nanoparticles loaded with saffron;

[0078] 5) Rotating the obtained saffron-loaded albumin nanoparticle suspension at 60° C. for 60 minutes in vacuum to remove the organic phase;

[0079] 6) The saffron-loaded albumin nanoparticle suspension was pa