Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof
An antibacterial peptide, rice shrimp technology, applied in the biological field, can solve the problems of shrimp and crab anorexia, muscle white turbidity, body surface ulcer, etc., and achieve the effect of reducing mortality and improving antibacterial ability.
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[0032] Zhejiang rice prawns are purchased from the Zhejiang aquatic product market. They are about 1.5-2.5cm in length and weigh about 50-100mg each. They are healthy and vigorous. The rice prawns are purchased and kept in the laboratory. Water aeration, the temperature is about 26-28°C. Shrimp food was fed twice a day, and fresh aeration water was changed every two days.
[0033] Short distance: time is 3s, 1000rpm.
[0034] Bursicon-α has the DNA sequence described in SEQUENCE LISTING NO.1.
[0035] The Bursa protein has the amino acid sequence described in SEQUENCE LISTING NO.2.
[0036] Bursicon-β has the DNA sequence described in SEQUENCE LISTING NO.3.
[0037] The Bursβ protein has the amino acid sequence described in SEQUENCE LISTING NO.4.
[0038] The technical solution of the present invention will be further described below in conjunction with specific embodiments.
[0039] Step 1, Trizol method to extract total RNA
[0040] Take 1.5ml enzyme-free EP tube and place it in liquid n
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