Production method of antibacterial peptide

[0018] Example 1

[0019] A method for producing antimicrobial peptides, the specific production steps are as follows:

[0020] S1. Put the bacteria expressed in the form of fusion protein during the preparation of antimicrobial peptides into a 300L seed tank for seed culture. After the bacteria reaches the conditions for subsequent culture inoculation, they are transferred to a 3m3 fermentor for fermentation, and the bacteria are subsequently cultured and inoculated The conditions are 6 hours of seed age, pH 5.6, OD 6.0; the end conditions of the fermentation culture of the strain are: 18 hours cycle, pH 5.9, OD 12.7; then transfer the fermentation mash to the transfer tank, and wait for the mash When the temperature in the transfer tank drops to 20-25℃, keep the mash at this temperature and let it stand for 16 hours in the transfer tank. After the standing is over, add diatomaceous earth to the transfer tank, and the amount of diatomite added is according to Add 4% of the volume of

[0022] Example 2

[0023] A method for producing antibacterial peptides, the specific production steps are as follows:

[0024] S1. Put the bacteria expressed in the form of fusion protein during the preparation of antimicrobial peptides into a 3000L seed tank for seed culture. After the bacteria reaches the conditions for subsequent culture inoculation, they are transferred to a 20m3 fermentor for fermentation, and the bacteria are subsequently cultured and inoculated The conditions are 6 hours of seed age, pH 5.6, OD 6.0; the end conditions of the fermentation culture of the bacteria are: 18 hours cycle, pH 5.8, OD 12.1; then transfer the fermentation mash to the transfer tank, and wait for the mash When the temperature in the transfer tank drops to 20-25℃, keep the mash at this temperature and let stand for 20 hours in the transfer tank. After the standing is finished, add diatomaceous earth to the transfer tank. The amount of diatomaceous earth added is according to Add 3% of the