Haploid molecular marker for identifying characteristic bloodline of Tibetan chicken and application of haplotype molecular marker

[0019] 1. Collection of experimental samples

[0020] According to the data released by the Tibet Animal Husbandry Bureau in 2007: Tibetan chickens are distributed in Shigatse and Shannan, each accounting for 30%, and Lhasa and Nyingchi each accounting for 15%. 115 Tibetan chickens with different feather colors from light to dark, 19 Lhasa white chickens, 98 local chickens and 45 red jungle chickens obtained by crossing the Tibetan chicken female parent with the Laihang chicken male parent were used as experimental materials. The specific information is shown in the table. 1 described.

[0021] Table 1 277 materials for experimental chickens

[0022]

[0023] 2. Genomic DNA extraction

[0024] Using the blood sample as the material, the genomic DNA was extracted using the QIAGEN DNA kit. The detailed steps are as follows:

[0025] (1) Add 20 μL of proteinase K to a 1.5 mL or 2 mL microcentrifuge tube, add 10 μL of anticoagulated blood, and then add 190 μL of PBS;

[0026]