Recombinant human fibronectin fragment for cell culture

[0042]The protein fragment of the present invention having amino acid sequence (SEQ ID NO: 3) was produced using a commercially available service. This protein fragment was then added on a surface to modify the surface. The surface of a cell culture vessel was modified by a protein fragment of the present invention using well established techniques known to a skilled artisan. In brief, covalent immobilization of a protein fragment was done by EDC / NHS Chemistry, protein fragment solution was added onto the surface followed by an incubation and wash step. Alternatively, for passive adsorption of a protein fragment, the protein fragment was diluted in phosphate buffered saline and the resultant solution applied to a tissue culture surface. Incubation was allowed at room temperature for at least 2 hrs after which the surface was washed and the tissue culture surface used. For human embryonic stem cells and induced pluripotent stem cells, tissue culture treated surface was coated for 2 hr