The invention provides a preparation method of Guizhou high-fragrance black tea. The preparation method comprises the following steps: picking tea leaves; soaking tea leaves; spreading the tea leaves;twisting the tea leaves; fermenting the tea leaves; shaping the tea leaves; drying the tea leaves. According to the preparation method of Guizhou high-fragrance black tea, based on the prior art, a soaking and washing step is added, and twisting is carried out under a low-temperature condition, so that deactivation of active enzyme due to high-temperature fermentation can be avoided, and the fermentation activity is higher; in addition, according to the fermentation properties of the tea leaves, three-step fermentation is adopted, and the prepared tea leaves have the advantages that the colorof made tea is ruddy, the tea is more red and dense in color and is more bright-colored, the fragrant smell is heavier and mellower and is more lasting, the taste is mellower, more smooth, and more dense, and the bottom parts of the tea leaves are more red and brighter.