The invention relates to a miRNA (micro Ribonucleic Acid) marker related to a pig hair-free character and application of the miRNA marker. The miRNA marker is miRNA-29a-5p, the sequence of the miRNA-29a-5p is shown as SEQ ID NO.1, the precursor sequence of the miRNA-29a-5p is shown as SEQ ID NO.2, and the
binding site of the miRNA-29a-5p and a
target gene EDAR is shown as SEQ ID NO.3; and when the
gene expression quantity of the mature miRNA-29a-5p in the pig
embryo skin tissue is lower than 3, the pig is judged as a hairy pig, and when the
gene expression quantity of the mature miRNA-29a-5p in the pig
embryo skin tissue is higher than 30, the pig is judged as a hair-free pig. The miRNA marker related to the pig hair-free character can be applied to identification and breeding of hair-free pigs, and the hair-free pigs identified and bred according to the technical scheme are applied to the fields of
medicine and scientific research.