4 results about "Drought tolerance" patented technology

The invention discloses a method for screening a drought-resistant and saline-alkaline-tolerant cotton variety, and relates to the technical field of agriculture. The method for screening the drought-resistant and saline-alkaline-tolerant cotton variety comprises the steps of manufacturing artificial saline-alkali land, preparing cotton seeds, cultivating cotton, detecting drought tolerance of thecotton and detecting the alkali tolerance of the cotton; the cotton variety with the highest drought resistance and highest saline-alkaline tolerance is obtained through actual cultivation, the defects of wrong selection and missing selection can be overcome, and seed selection is more convincing.

The invention relates to a design method for the evaluation test of the high and stable dry-land yield of a sugarcane breed. A dry sloping field which has the annual rainfall below the natural drought condition suitable for the growth of sugarcane is taken as an evaluation test field; a thick film is buried with the depth of 1m around the test field; no artificial irrigation is carried out during the whole growing period of sugarcane; the breed is compared with generalized breeds with good, high and stable dry-land yield; the test period is two years for newly-planted sugarcane and one year for perennial-root sugarcane; and the plot arrangement for two times of newly-planted sugarcane adopts the completely randomized block design. The method ensures the full play of the drought escape and resistance of the tested breed through the three measures of artificially selecting the naturally drought area as the test field, separating water around the test field and carrying out no artificial irrigation. Through adopting the breeds with good, high and stable dry-land yield as comparison breeds and carrying out the two-year newly-planted and one-year perennial-root sugarcane test, the method ensures the full play of the tested breed with high and stable dry-land yield and has high comparability.

The invention relates to a method for testing drought tolerances of vigna unguiculata type I and vigna unguiculata type II by fluorescence quantitative PCR, diagnostic genes and primers. According to the method, sequences of the tested diagnostic genes are shown as in SEQIDNO: 1; the fluorescence quantitative PCR primers have the sequences as follows: a forward primer: 5'-TGGATTGTGGTGGACATAC-3' and a downstream primer: 5'-GGTTCCTTCTGAGCATTGA-3'. The method, the diagnostic genes and the primers have the advantages that significant differentially expressed genes in materials of drought tolerances of the vigna unguiculata type I and the vigna unguiculata type II provide a diagnostic gene tool for distinguishing the drought tolerances of different types of vigna unguiculata specifically. In addition, direct technical support is provided for testing drought tolerance types of different breeding parent materials by real-time fluorescence quantitative PCR rapidly and conveniently based on the primer sequences of the tolerance diagnostic genes of the vigna unguiculata type I and the vigna unguiculata type II.

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes an SIPR polypeptide.