The invention discloses bacillus velezensis D-1 as well as a preparation and application thereof. The name of the bacillus velezensis D-1 is bacillus velezensis D-1, the bacillus velezensis D-1 is preserved in the China General Microbiological Culture Collection Center on October 12, 2021, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.23579. The bacillus velezensis D-1 is named as bacillus velezensis D-1. The bacillus velezensis D-1 disclosed by the invention has a remarkable antagonistic effect on three kinds of fusarium causing taro dry rot, also has antagonistic activity on other important plant pathogenic fungi and pathogenic bacteria, and provides a new resource for biological prevention and control of various plant diseases in current agricultural production.