The invention discloses a composition for preventing and treating gill rot, enteritis and red skin disease of grass carps, and relates to the technical field of aquaculture. The composition is prepared from the following raw material medicines in parts by weight: 10 parts of rheum rhabarbarum, 10 parts of parslane herb, 10 parts of amur corktree bark, 10 parts of baikal skullcap root, 12 parts of toad venom, 8 parts of honeysuckle flower, 8 parts of fresh ginger and 15 parts of liquorice root; and the composition is prepared by grinding the raw material medicines into powder and mixing. The composition disclosed by the invention has the beneficial effects that the composition has significant effects on prevention and treatment of bacterial 'three old diseases' such as gill-rot, enteritis and red skin disease of the grass carps, is simple in use method, is convenient to administrate medicines into ponds, and is high in cure rate and rapid in recovery; pathogenic bacteria cannot have medicine resistance to the composition, and the environmental pollution cannot be caused to an aquaculture water body; and the composition is cheap in medicine and low in treatment cost.