The invention discloses a method for performing
cross breeding on water chestnut and Dongkui waxberry for waxberry cultivating variety. The method comprises the following steps: by taking water chestnut as a female parent and taking male flower of Dongkui waxberry as a male parent, pollinating during water chestnut full-blossom period; collecting seeds after the fruits are ripened, storing the seeds at the temperature of 5 DEG C, seeding to obtain F1 plants; and screening true
hybrid plants from the F1 plants. Intervarietal
cross breeding between the waxberry cultivating varieties such as water chestnut and Dongkui waxberry is realized for the first time, true
hybrid plants with certain genotypes are obtained, and a novel technological approach is provided for breeding novel waxberry variety. Moreover, the filial generation is subjected to
hybrid identification by amplifying specific expression SSR markers of the F1 generation, the identification process is simple and rapid and the cost is low, the identification result is stable and reliable, and the gap of the molecular biological
identification technology of waxberry intervarietal hybridization is filled.