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5 results about "Cross breeding" patented technology

Blowing pollinator and method for using same to carry out blowing pollination

The invention relates to the field of cross breeding, in particular to a blowing pollinator and a method for using the same to carry out blowing pollination. The blowing pollinator comprises a fan and an air guide; the air outlet of the fan is connected with the air guide, and the air outlet end of the air guide is connected with two air nozzles; each air nozzle is an adjustable air nozzle, and can adjust the angle between the jetted airflow and the horizontal plane; each air nozzle is perpendicular to the air outlet end of the air guide, i.e., the flowing direction is changed by 90 degrees after the airflow enters the air nozzle via the air guide; the two air nozzles respectively face up at the left and the right of the air outlet end of the air guide; and the fan is connected with a storage battery for supplying power and a flow velocity regulator for regulating the velocity of the airflow. The blowing pollinator and the blowing pollination method provided by the invention can utilize the airflow to directly blow the mature pollen of male parents into the air over a male parent area, the pollen then spontaneously falls to finish the pollination process, and thereby the setting rate of the cross breeding can be remarkably increased.
Owner:SICHUAN CHUANLONG TRACTORS MFG CO LTD

Method for performing cross breeding on water chestnut and Dongkui waxberry for waxberry cultivating variety

ActiveCN104026001AAchieve hybridizationWidely used in germplasm innovationPlant genotype modificationWater ChestnutsGenotype determination
The invention discloses a method for performing cross breeding on water chestnut and Dongkui waxberry for waxberry cultivating variety. The method comprises the following steps: by taking water chestnut as a female parent and taking male flower of Dongkui waxberry as a male parent, pollinating during water chestnut full-blossom period; collecting seeds after the fruits are ripened, storing the seeds at the temperature of 5 DEG C, seeding to obtain F1 plants; and screening true hybrid plants from the F1 plants. Intervarietal cross breeding between the waxberry cultivating varieties such as water chestnut and Dongkui waxberry is realized for the first time, true hybrid plants with certain genotypes are obtained, and a novel technological approach is provided for breeding novel waxberry variety. Moreover, the filial generation is subjected to hybrid identification by amplifying specific expression SSR markers of the F1 generation, the identification process is simple and rapid and the cost is low, the identification result is stable and reliable, and the gap of the molecular biological identification technology of waxberry intervarietal hybridization is filled.
Owner:ZHEJIANG UNIV +1

Functional markers of maize recessive kernel male sterile mutant gene ms33 and application of functional markers

The invention relates to functional markers of a maize kernel male sterile gene ms33 as well as a development method and application of the functional markers. The invention designs five functional markers includes, but is not limited to, the functional markers ms33-IN1 and ms33-IN2 for a mutant ms33-6029 as well as ms33-IN3, ms33-IN4, ms33-IN5 for the mutant ms33-6038, and the functional markerscan be used for specifically detecting the maize mutant ms33-6029 or ms33-6038 as well as the mutant gene ms33 in a maize sterile material transformed therefrom. By utilizing the five functional markers or other similar functional markers involved in the invention, alleles of the kernel male sterile gene ms33 or a fertile gene Ms33 in a sample can be accurately and rapidly identified by PCR and agarose gel electrophoretic detection; the functional markers can be used for identification of male sterile lines and screening of target individuals, have important application values in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection, and are of great significance to improvements on maize cross breeding and seed production efficiency, guarantee of the quality of seed production and other aspects.
Owner:UNIV OF SCI & TECH BEIJING +1
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