16 results about "Molecular marker" patented technology

The invention belongs to the technical field of genetic engineering, in particular to specific molecular markers of sex chromosomes of Tilapia nilotica, wherein the nucleotide sequences of specific molecular markers NtY1 and NtY2 of a Y chromosome are respectively shown in SEQID No.1 and SEQID No.2, and the nucleotide sequence of a specific molecular marker NtX1 of a X chromosome is shown in SEQID No.3; in the invention, a genetic sex identification method of Tilapia nilotica is established by using the specific molecular markers of the sex chromosomes of the Nile tilapia, and through carrying out PCR amplification on any one or more than one of the specific molecular markers of the sex chromosomes, YY super-male fishes, XY milter and XX female fishes can be identified economically, quickly and accurately, thus being beneficial to realizing the large-scale production of holandric Tilapia nilotica fries through YY super-male fish breeding routes, obviously improving the breeding production of Tilapia nilotica, reducing the breeding cost, and improving the economic benefit.

The invention discloses valid combinations of 8 novel SSR molecular markers and 2 known SSR molecular markers. The valid combinations are applied to strain identification. DNAs of 22 specific strains are taken as templates, 10 pairs of SSR primers are respectively utilized for carrying out PCR amplification, each pair of SSR primers is subjected to acrylamide gel electrophoresis after the PCR amplification, banding pattern statistic analysis is carried out according to all electrophoretic bands amplified from 22 samples, and therefore, a standard banding pattern of each pair of primers is determined; then the standard banding patterns of 10 pairs of primers of each sample are combined, and molecular identification cards of the 22 different strains of agaricus bisporus are constructed. According to an agaricus bisporus SSR molecular marker specific primer system and an application thereof, the strain identification effect of agaricus bisporus is improved; the agaricus bisporus SSR molecular marker specific primer system and the application are significant for the resource identification, the protection, the development and the utilization of agaricus bisporus.

The invention discloses a method for judging the sex of trachinotus ovatus at an early stage. Young trachinotus ovatus is selected, the left body wall is cut off, sex gland is found out, characteristics of the sex gland are observed in situ, the sex gland is taken out and dissected, the characteristics of the dissection face of the sex gland are observed, and the sex of the trachinotus ovatus is judged by analyzing the characteristics of the sex gland According to the method, the instrument is simple, operation is rapid, the result is accurate, toxic chemical agents are not used, the method for judging the sex of the trachinotus ovatus at the early stage is convenient to carry out efficient, and reliable materials can be provided for molecular marker research for further searching for sex specialty of the trachinotus ovatus.

The invention discloses a drought-resistant related SSR sequence derived from abnormal cotton and application thereof. The invention provides application of a specific DNA fragment or related biomaterials thereof in cultivation of drought-resistant cotton varieties or strains or improvement of the drought resistance of cotton. The specific DNA fragment is a fragment located on chromosome 5 of abnormal cotton and at least containing a sequence from an SSR molecular marker JAAS6365 (SEQ ID No. 1) to an SSR molecular marker JAAS5604 (SEQ ID No. 2). The related biomaterials are a vector, an expression cassette, recombinant bacteria or transgenic cell line containing the specific DNA fragment. The chromosome fragment provided by the invention has important application value in drought-resistantcotton breeding, and at the same time the SSR molecular marker developed by the invention provides a molecular basis for cultivation of drought-resistant cotton varieties that can be applied to production.

The invention belongs to the technical field of molecular biology and discloses a molecular marker for identifying rice grain length characters, an identification method and application. The molecular marker is GS3-TPAP and comprises GS3-TPAP-O-F with the nucleotide sequence shown as SEQ ID NO.1, GS3-TPAP-O-R with the nucleotide sequence shown as SEQ ID NO.2, GS3-TPAP-I-F with the nucleotide sequence shown as SEQ ID NO.3, and GS3-TPAP-I-R with the nucleotide sequence shown as SEQ ID NO.4. The molecular marker can be used for directly identifying two allelotypes of the rice grain length characters of the GS3 gene, the accuracy reaches 100%, the two allelotypes can be distinguished well, influences of environmental conditions are avoided, and the identifying method is low in consumption and suitable for large-scale application and popularization.

The invention relates to the technical field of biology, in particular to application of ICOSL (Inducible Costimulator Ligand) protein in preparation of a kit used for breast cancer prognosis evaluation. According to the invention, the immunohistochemistry technology and a semi-quantitative analysis method of a staining result are employed to analyze the expression level of the ICOSL protein in atumor tissue, and combine with post-operative follow-up information to determine whether the ICOSL protein expression has correlation with the prognosis of a breast cancer patient or not, wherein theICOSL protein can be used for preparing a protein molecular marker for determining the prognosis of the breast cancer patient to have important guiding significance for post-operative monitoring and sequential treatment of the breast cancer patients.

The invention provides a method for quickly identifying idioplasm of capsicum frutescrens L. by using an SSR (simple sequence repeat) molecular marker, and belongs to the technical fields of plant molecular marker-assisted idioplasm resource identification and seed breeding. The SSR molecular marker can be obtained by amplifying a primer shown in SEQ ID NO.1 to 2. The SSR molecular marker has theadvantages that the idioplasm of C.frutescens and the idioplasm of C.annuum can be distinguished; the operation is simple, the recurrence is good, and the accuracy is high; the disadvantage of difficulty in distinguishing the idioplasm of C.frutescens and the idioplasm of C.annuum in the phenotype identification method can be effectively overcome; the important function is realized in the pepper idioplasm resource identification and the molecular marker-assisted seed breeding.

The invention discloses a molecular marker of maize transcription factor ZmPIF3 transgenic rice and application of the molecular marker and belongs to the field of crop genetic breeding. A maize transcription factor ZmPIF3 transgenic rice material which is obtained already has obvious water-saving drought-enduring phenotype. According to a nucleotide sequence of maize transcription factor ZmPIF3 gene, a primer of the molecular marker is designed in CDS, PCR technology is applied to acquire DNA segments of ZmPIF3 and sequence the DNA segments, and the primer of the molecular marker is obtained according to specific segment screening of ZmPIF3. The molecular marker is high in repeatability, stable in amplification, simple and convenient to operate and low in cost and can be used for detecting maize ZmPIF3 genes in rice background, and a solid foundation is laid for molecular marker assisted selection in the future by utilizing ZmPIF3 gene transgenic rice.

The invention provides identification and application of pig CD4 gene function mutation site molecular breeding marker, and particularly provides four function mutation sites of CD4 gene. The four function mutation sites have impact on expression level, membrane localization and immune level of the CD4 gene. Four key mutation sites of the molecular marker are characterized by having mutation at basic groups of 193rd (A/G), 195th (G/C), 196th (A/G) and 202nd (C/G) of a CDS area. A new molecular marker is provided for marker assisted selection of pig immune characters.

The present invention describes systems and methods for formulating anti-tamper label and security tape adhesive chemistry with nucleic acid identifier molecular markers to authenticate the tape or label affixed to an article.

The invention belongs to the field of molecular marker-assisted breeding of aquatic animals, and particularly relates to a group of prawn growth related SNP markers and an application thereof in breeding. The growth related markers are shown as sequences SNP3085_190487, SNP1678_860109, SNP3406_465799 and SNP2570_399791, the marked dominant genotypes of the growth related markers are CC, GG, GG andCC respectively, and the growth speed of prawns can be remarkably increased by performing genotyping and marker selection on one or more SNP markers. The invention also discloses an application of the growth related markers in genetic breeding. Compared with a traditional breeding method, the method has the advantages that character-related molecular markers are used for assisting in breeding, and accuracy and high efficiency are achieved; and besides, the multiple markers can be used in a combined mode, rapid improvement of growth characters is achieved through marker polymerization, and themethod has wide application prospects in cultivation of high-yield varieties.

The invention relates to an SSR (Simple Sequence Repeat) marker primer for black camellia hybrid identification. The primer has a primer number SSR31, an upstream primer: 5 '-CAAGGCAGAGTAGGCTGAGG-3', a downstream primer: 5 '-AAAAGGAAGGGAAAGTCA-3', a repetitive unit: (AGC) 7 and a fragment size of 212-273; the primer number is SSR48, the upstream primer is 5 '-GTTGGAGGTTTTTCAGCCAC-3', the downstream primer is 5 '-ATTGAAAGTCGCTGCTTCGT-3', the repetitive unit is (CCG) 7, and the fragment size is 249 to 300; the primer number is SSR250, the upstream primer is 5 '-ACCTCAGCTTCCATAGA-3', the downstream primer is 5 '-ATCTTGACGGGCGTAACATA-3', the repetitive unit is (CCG) 5, and the size of the fragment is 261 to 315. The invention also discloses a method for detecting the content of the CCG in the sample. The SSR marker disclosed by the invention is good in repeatability and strong in specificity; the black camellia hybrid is effectively identified by using an SSR molecular marker technology, the relationship between the black camellia hybrid and parent heritable variation is defined, and rapid identification and early selection of the black camellia hybrid offspring are realized.