Application of TROAP in preparation of renal cell carcinoma prognosis product and therapeutic drug

[0035] Correlation experiment between TROAP and renal cancer.

[0036] The protein expression level of TROAP in 20 pairs of renal cancer and corresponding renal normal tissues was detected by immunohistochemistry, and the results showed that TROAP was significantly highly expressed in renal cancer tissues; subsequent subgroup analysis of renal cancer grades indicated that as the grade increased, the tumor The expression of TROAP in tissues also showed an increasing trend, and the results were statistically different ( figure 1 ), the results suggest that TROAP may be closely related to the occurrence and development of RCC.

[0037] see figure 1 .Immunohistochemical verification of the expression of TROAP in clear cell renal cell carcinoma.

[0038] Immunohistochemical (IHS) results showed:

[0039] A.B.C.TROAP was highly expressed in renal cancer tissues, and there was a statistically significant difference (P<0.001);

[0040] D-H. The higher the clinical grade of renal c...

[0042] In vitro and in vivo experiments showed that knocking down TROAP could inhibit the proliferation, migration and invasion of renal cancer cells.

[0043] Because TROAP is closely related to the clinical practice of renal cell carcinoma, its function in the progression of renal cell carcinoma was further explored. The commonly used kidney cancer cell lines 786-0 and ACHN were selected, and the lentivirus interference technology was used to knock down TROAP ( figure 2 ), functional experiments include MTT cell proliferation, apoptosis, cell scratch healing and Transwell invasion in vitro. Knockdown of TROAP can significantly inhibit the growth of 786-0 and ACHN cells ( image 3 ); Knockdown of TROAP significantly increased the percentage of apoptosis in 786-0 and ACHN cells ( Figure 4 ); Knockdown of TROAP significantly reduced the scratch healing ability of 786-0 and ACHN cells ( Figure 5 ); Knockdown of TROAP significantly inhibited the invasion ability of 786-0 an...

[0052] In vivo subcutaneous tumor formation experiment in nude mice.

[0053] The present invention carried out subcutaneous tumor formation experiments in nude mice in vivo, and subcutaneously inoculated ACHN cells. The results showed that the tumor growth of the TROAP knockdown group was slow, and the final tumor volume was significantly smaller than that of the control group ( Figure 7 ).

[0054] see Figure 7 . Subcutaneous tumor growth experiment in nude mice. Subcutaneous tumor growth was inhibited after TROAP interference.

[0055] A: 10 pairs of nude mice and their subcutaneous tumors.

[0056] B: The growth curve of subcutaneous tumors in nude mice, showing that the growth of subcutaneous tumors in the TROAP interference group was significantly inhibited.

[0057] C: The average volume of subcutaneous tumors in nude mice after 4 weeks, showing that the volume of the TROAP interference group was significantly lower than that of the control group.