4 results about "Intron" patented technology

The present invention relates to methods and devices for identifying and quantifying, including low abundance, nucleotide base mutations, insertions, deletions, translocations, splice variants, miRNA variants, alternative transcripts, alternative start sites, alternative coding sequences, alternative non-coding sequences, alternative splicings, exon insertions, exon deletions, intron insertions, or other rearrangement at the genome level and/or methylated nucleotide bases.

The invention provides a genome editing method based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) system and belongs to the technical field of gene editing. The genome editing method provided by the invention utilizes introns to express guide RNA molecules in the CRISPR genome editing system and adopts a principle that one or more than one tRNA-gRNA or crRNA series unitsare arranged in the introns of an encoded gene, and after the introns form a fusion gene with Cas9 or Cpf1, a promoter can be used for driving. The genome editing method provided by the invention hasthe benefits that a cellular endogenous mRNA splicing system and a cellular endogenous tRNA processing system are skillfully utilized, and other elements are not required to be added, so that not onlyis the safety higher, but also more simplicity can be realized; the synchronous expression of a plurality of guide RNAs with the Cas9 or the Cpf1 is realized by utilizing the one promoter, so that anexisting CRISPR editing system is simplified, moreover, the efficiency and the ability of simultaneously editing multiple target points of the CRISPR editing system are improved.

Present invention relates to the Biomarkers for detecting high altitude adaptation and hypoxia responsiveness and the method thereof. The invention specifically relates to the Gene variants SNPIDs rs479200 and rs480902 in the first intron of EGLN1 (Prolyl Hydroxylase 2) gene as biomarkers for adaptation to high altitude and predisposition for high altitude pulmonary edema and hypoxia responsiveness using a novel integrative approach of phenotyping concepts of Ayurveda with population genetics, and disease genomics. More specifically, the C allele of SNP ID rs480902 and T allele of rs479200 of EGLN1 gene is more frequent in patients of HAPE and nearly absent in native highlanders. The present invention also provides primers and methods suitable for the detection of these allelic variants for the prediction of individual's adaptability to high altitude and hypoxia and/or the genetic analysis of the EGLN1 gene in a population.

The invention relates to identification and application of sugarcane SPSB gene isotype 2, and belongs to the technical field of plant molecular breeding. A cDNA sequence of high-sugar sugarcane YZ14-401 is used as a template, SPSB2351 is used as a primer for PCR amplification, a haplotype of an SPSB gene is screened through sequencing, a fifth intron part retention sequence exists between a fifthexon and a sixth exon, and sequences at two ends of the primer are completely the same as a CDS sequence of the SPSB gene. The SPSB gene isotype 2 can be used for designing a specific primer in a fifth intron retention section, identifying the expression of the isotype 2 and carrying out molecular marker-assisted selection. The sequence required for identifying the gene expression level of the SPSB gene isotype 2 is provided for sugarcane sugar trait marker-assisted breeding; tracking, identification and expression abundance detection of different haplotypes or isotypes of the SPSB gene in sugarcane filial generations are facilitated; and the application has important significance in rapid establishment of sugarcane germplasm with excellent heredity.