Identification and application of sugarcane SPSB gene isotype 2

[0041] Cloning and sequencing analysis of the cDNA sequence of sugarcane SPSB gene of embodiment 1

[0042] 1. Sample RNA extraction and cDNA synthesis

[0043]In the present invention, sugarcane YZ14-401 leaves at the mature stage are used as detection objects, and the total RNA is extracted with the EasyPurePlant RNA Kit provided by Quanshijin Company, and finally dissolved in sterilized ultrapure water, and stored at 4°C after detection. The OD value of the RNA sample at 260nm and 280nm was measured with a UV photometer. Calculate the RNA content and the ratio of OD260 / OD280 to evaluate the quality of RNA extraction; after the RNA detection is completed, take out a certain amount and dilute it to 10ng / μL for the synthesis template of cDNA. First-Strand cDNA Synthesis SuperMix kit was used to synthesize cDNA.

[0044] 2. Primer design

[0045] The published SPSB gene sequence was obtained from NCBI, and the GenBank ID was JN584485. Primer5.0 software was used to design

[0055] Cloning and sequencing analysis of the cDNA sequence of the sugarcane SPSB gene of embodiment 2

[0056] 1. Sample RNA extraction and cDNA synthesis

[0057] In the present invention, sugarcane YZ14-401 leaves at the mature stage are used as detection objects, and the total RNA is extracted with the EasyPurePlant RNA Kit provided by Quanshijin Company, and finally dissolved in sterilized ultrapure water, and stored at 4°C after detection. The OD value of the RNA sample at 260nm and 280nm was measured with a UV photometer. Calculate the RNA content and the ratio of OD260 / OD280 to evaluate the quality of RNA extraction; after the RNA detection is completed, take out a certain amount and dilute it to 10ng / μL for the synthesis template of cDNA. First-Strand cDNA Synthesis SuperMix kit was used to synthesize cDNA.

[0058] 2. Primer design

[0059] The published SPSB gene sequence was obtained from NCBI, and the GenBank ID was JN584485. Primer5.0 software was used to de

[0066] Example 3 Cloning and sequencing analysis of sugarcane SPSB gene cDNA sequence

[0067] 1. Sample RNA extraction and cDNA synthesis

[0068] In the present invention, sugarcane YZ14-401 leaves at the mature stage are used as detection objects, and the total RNA is extracted with the EasyPurePlant RNA Kit provided by Quanshijin Company, and finally dissolved in sterilized ultrapure water, and stored at 4°C after detection. The OD value of the RNA sample at 260nm and 280nm was measured with a UV photometer. Calculate the RNA content and the ratio of OD260 / OD280 to evaluate the quality of RNA extraction; after the RNA detection is completed, take out a certain amount and dilute it to 10ng / μL for the synthesis template of cDNA. First-Strand cDNA Synthesis SuperMix kit was used to synthesize cDNA.

[0069] 2. Primer design

[0070] The published SPSB gene sequence was obtained from NCBI, and the GenBank ID was JN584485. Primer5.0 software was used to design primers in t