3 results about "Penicillin" patented technology

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention belongs to the technical field of animal breeding, and particularly relates to animal fresh semen low-temperature diluent as well as a preparation method and application thereof. The animal fresh semen low-temperature diluent is prepared from the following ingredients: glucose, sodium citrate, penicillin, streptomycin, enrofloxacin, yolk, defatted milk, vitamin A, vitamin D, vitaminE and water. The diluent has the advantages that Australian white sheep semen is stored for 1 to 2 days in a liquid state form under the condition of low temperature being 0 to 5 DEG C; the artificialinsemination requirement can be reached; the fresh semen storage quality is improved, so that the utilization rate of excellent rams is improved; high nanny goat conception rate is obtained; the problem of long-distance transportation after excellent ram semen collection is solved; the manufacturing method of the diluent is simple; the raw material cost is low; the operability is high; the large-scale popularization and application in the production is convenient.

The present invention relates to a purple Chinese yam fruit juice preservation liquid which is prepared from tea polyphenols, citric acids, sodium fluoride and penicillin at mass ratios of 3 : 5 : 1 : 1. The application of the fruit juice preservation is as follows: 1) peeling, washing, and dicing purple Chinese yams, adding sugar and water, and boiling the mixture; 2) putting the cooked purple Chinese yams and sugar juice into a blender, squeezing, filtering, and removing residues; 3) adding pectin enzyme to conduct hydrolysis; 4) adding the preservation liquid; 5) conducting an ultra high-temperature sterilization and aseptic filling; and 6) pumping the air in a container, and filling with an inert gas, and thereby preserving and storing at room temperature. The preservation liquid can effectively conduct color retention and preservation for the purple Chinese yam juice, and the prepared preservation liquid is natural and non-toxic, and has no harm to human body health.