The invention discloses a targeting probe for photodynamic therapy and a preparation method. The targeting probe includes a synthetic drug-loaded microbubble, a targeting molecule small peptide LHRH (gonadotropin-releasing hormone) connected to it, and a photosensitizer ZnPc (zinc phthalocyanine) wrapped in it. Compared with the traditional photosensitizers, the present invention overcomes the disadvantages of the existing photosensitizers, which are non-targeting, difficult to be enriched to reach an effective concentration, easy to agglomerate, difficult to deliver to the tumor site, and mostly limited to the tumor site in the epidermis or superficial tissue area, providing A targeted multimode probe with ultrasonic signal, photoacoustic signal and fluorescent signal for photodynamic therapy with high targeting, strong tissue penetration, good stability and strong specificity.