The invention discloses a long-chain non-coding
RNA and an application of the long-chain non-coding
RNA in diagnosis and treatment of
hepatocellular carcinoma. The long-chain non-coding
RNA is lncRNA MCM3AP-AS1, and the
nucleotide sequence of the lncRNA MCM3AP-AS1 is as shown in Seq ID NO. 1. It is found for the first time that lncRNA MCM3AP-AS1 can be used as a
prognosis biomarker and a
drug treatment target of
hepatocellular carcinoma, lncRNA MCM3AP-AS1 is obviously up-regulated in
hepatocellular carcinoma tissues and
cell lines, the prognosis of HCC patients overexpressing lncRNA MCM3AP-AS1 is poorer, meanwhile, lncRNA MCM3AP-AS1 has good hepatocellular
carcinoma prognosis ability, and through in-vitro and in-vivo experiments, it is proved that the lncRNA MCM3AP-AS1 exists in
cytoplasm, and the proliferation of hepatocellular
carcinoma can be inhibited by knocking down the lncRNA MCM3AP-AS1, such that the lncRNA MCM3AP-AS1 can provide the
cancer promoting effect in the occurrence and development process of hepatocellular
carcinoma, and can be adopted as the novel treatment target of hepatocellular carcinoma.