3 results about "RNA extraction" patented technology

The invention provides a method for extracting extracellular RNA from the seminal plasma, which comprises the following steps of: collecting and separating the seminal plasma, carrying out denaturing liquid processing on the seminal plasma twice, precipitating and washing RNA with isopropanol and ethanol and the like. In the method, the biochemistrical characteristics of the seminal plasma and the characteristics of extracellular RNA are combined, RNA extracting reagents are selected and combined with pertinence, N-sarcosyl is added in the denaturing liquid aiming at the rich protein contained in the seminal plasma so as to promote the denaturing effect of the guanidine thiocyanate on protein, and Triton x-100 is added in the denaturing liquid aiming at the characteristic that the extracellular RNA combines with certain substances so as to be beneficial to the separation of the RNA from the combined substances. The method has low cost, reliable result, easy promotion and application, high extracellular RNA extracted content and good quality, is a noninvasive means, can substitute the traditional puncture method and is used for the genital organs gene description study and organ function detection and evaluation.