The invention relates to a gene chip for detecting transgenic plants and products containing screened gene NOS, belonging to the technical field of biological engineering. The chip comprises a glass slide with an aldehyde group modified surface, a screened gene NOS probe coated on the glass slide in an array mode, and a positive control, a hybridization positive control, a positive quality control probe, a negative quality control probe and a blank control which are fixed by nucleic acid, wherein the sequence of the screened gene NOS probe is NH2-(CH2)6-O-Poly (dT)15-AAGCATGTAATAATTAACATGTAATGCATGACGTTATTTA. The gene chip for detection of the screened gene NOS of the transgenic plants and the products of the invention can carry out specific detection on the screened gene NOS of the transgenic plants and the products thereof and has high specificity.