Gene chip for detecting transgenic plant and products containing screened gene NOS and application thereof

A technology for genetically modified plants and screening genes, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, fluorescence/phosphorescence, etc. It can solve the problems of patent reports on gene chip detection technology that have not yet found NOS genes, and improve supervision. Level, good specificity, high sensitivity effect

Inactive Publication Date: 2010-09-08
山东鲁保科技开发有限公司
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  • Abstract
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AI Technical Summary

Benefits of technology

This patented technology allows researchers to test different types of DNA sequences that are involved with plant growth or development without having to physically touch them directly through various methods like microscopies or fluorescence techniques. It also includes an easy way to compare these results against each other's performance compared to previous experiments.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the efficiency and precision of detecting DNA modifications during crop breeding processes while considering potential negative effects from these techniques like crossbreaking and reprocessing steps.

Method used

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  • Gene chip for detecting transgenic plant and products containing screened gene NOS and application thereof
  • Gene chip for detecting transgenic plant and products containing screened gene NOS and application thereof
  • Gene chip for detecting transgenic plant and products containing screened gene NOS and application thereof

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Embodiment 1

[0042] 1. Design of primers and probes

[0043] The primers were fluorescently labeled with Cy3-Amidite at the 5' end. The primers were synthesized by Shanghai Boya Biological Company, and the primers were diluted to 10 μmol / L for use.

[0044] Use Primer premier 5.0 and Oligo 6.0 software to design 40mer Oligo candidate probes according to the sequence of NOS. The general principles of design are: (1) have relatively similar TM values, and the fluctuation range is 5°C; (2) GC content is 45% ~55%, otherwise the background of non-specific hybridization will be increased; (3) the repeated single bases shall not exceed 4 consecutively; (4) the length of the paired bases of the most stable secondary structure of the probe molecule shall not be greater than 4bp. The probe was modified with aminohexane at the 5' end, and the amino group and the probe sequence were connected with a spacer poly(dT15), so that the final probe length was 55 bp. All probes were synthesized in Shanghai B

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Abstract

The invention relates to a gene chip for detecting transgenic plants and products containing screened gene NOS, belonging to the technical field of biological engineering. The chip comprises a glass slide with an aldehyde group modified surface, a screened gene NOS probe coated on the glass slide in an array mode, and a positive control, a hybridization positive control, a positive quality control probe, a negative quality control probe and a blank control which are fixed by nucleic acid, wherein the sequence of the screened gene NOS probe is NH2-(CH2)6-O-Poly (dT)15-AAGCATGTAATAATTAACATGTAATGCATGACGTTATTTA. The gene chip for detection of the screened gene NOS of the transgenic plants and the products of the invention can carry out specific detection on the screened gene NOS of the transgenic plants and the products thereof and has high specificity.

Description

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Claims

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Application Information

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Owner 山东鲁保科技开发有限公司
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