3 results about "Suspension culture" patented technology

The invention relates to the field of biotechnologies, in particular to a passage method of pluripotent stem cells and application thereof. The method includes: shifting quantitative pluripotent stem cell balls into a medium, and mixing them evenly; making use of a liquid shifter to suck the pluripotent stem cell balls mixed in the medium, and making use of the pressure generated by the liquid shifter to perform extrusion so as to make the balls pass a cell sieve of 30-70 micrometers, thus forming more pluripotent stem cell small block masses mixed in the medium; and transferring the pluripotent stem cell small block masses mixed in the medium into a new culture dish, thus completing passage. The method is used for passage of vertebrate embryonic stem cells and induced pluripotent stem cells. The method provided in the invention is suitable for pluripotent stem cell balls formed by suspension culture, and is a novel passage method with no need for enzymatic digestion. After passage by the method, pluripotent stem cell cloned balls have a high survival rate up to 90%, and each passage ratio can reach 1:8-1:10, so that the method can meet the large-scale volume production needs of stem cells.

The invention discloses a bovine kidney cell capable of being subjected to suspension culture, a suspension culture method for the bovine kidney cell and application thereof. The bovine kidney cell, the suspension culture method for the bovine kidney cell (MDBK) and the application have the advantages that a large quantity of manual operation for cell digestion, liquid change, frequent seed separation and the like can be omitted by the suspension culture method, the suspension culture method is simple, convenient and speedy, processes can be automated by the aid of reactor culture, the cell yield of the unit volume can be increased, and the technical difficult problem of difference between the traditional adherent culture cell batches can be solved; the bovine kidney cell (MDBK) and suspension culture cells of the bovine kidney cell can be used in the fields of culture of diversified sensitive viruses, production of antibody proteins, production of vaccine, examination of exogenous viruses of products and the like.

The invention discloses a method for inhibiting stemness of pancreatic cancer stem cells with miR-200a (micro ribonucleic acid-200a). The method comprises the steps of culturing a human pancreatic cancer cell line PANC-1, detecting and sorting the pancreatic cancer stem cells with a flow cytometer, conducting serum-free suspension culture on PANC-1 stem cell spheroid cells, and allowing miR-200a to over-express. The method is convenient to operate, good in effect and low in cost.