Preparing process and usage of grease of housefly larva and fatty acid thereof

A technology of fly maggot oil and fatty acid, which is applied in the field of bioengineering, can solve the problems of rarely developing health care products, and achieve the effect of not easy to oxidize and deteriorate, easy to absorb, and stable quality

Inactive Publication Date: 2007-08-08
沈荣法 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of product made from living flies called Maggella spp., which are tiny creatures found throughout nature or other environments where they live) was developed with this unique ingredient added without harming their healthy flora. It's safe because no chemical substances were used during production.

Problems solved by technology

This patented technical problem addressed by this patents relates to extracting ferrous acid (FCA) or other valuable nutrients found naturally within animal tissues such as seafood, while also avoids extraction with these types of lipase that contain sialic acid residues attached to them during processing steps like refining.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Using the fly maggots cultivated by the breeding method with the patent application number 200510060277.4 as raw materials, wash the fly maggots with water, dry them at a temperature of 40°C at a vacuum of 0.01Mpa until the water content in the fly maggots is 10%, crush them, and add 1 times its weight Petroleum ether, leached at 0°C for 5 hours, separated solid-liquid two phases, and the liquid phase was evaporated at 20°C under a vacuum of 0.01Mpa to recover petroleum ether to obtain fly maggot oil; In sodium pH6.0 buffer solution, add porcine pancreatic neutral lipase to reach 180 international units of enzyme activity per milliliter; in the buffer solution equipped with porcine pancreatic neutral lipase, add fly maggot oil, the difference between buffer solution and fly maggot oil The volume ratio was 1:0.5, kept at 38°C, stirred and reacted for 1.5 hours, stopped the reaction, separated statically, separated the oil phase, washed twice with an equal volume of water, an

Embodiment 2

[0017] Using the fly maggots cultivated by the breeding method with the patent application number 200510060277.4 as raw materials, wash the fly maggots with water, dry them at a vacuum degree of 0.003Mpa and a temperature of 30°C until the water content in the fly maggots is 13%, crush them, and add 10 times the weight of the fly maggots. Alkanes, leaching at 50°C for 1 hour, separating the solid and liquid phases, and evaporating the liquid phase at 30°C under a vacuum of 0.003Mpa to recover hexane to obtain fly maggot oil; In pH8.0 buffer solution, add yeast neutral lipase, reach per milliliter 200 enzyme activity international units; Add fly maggot oil in the buffer solution that is mixed with yeast neutral lipase, the volume ratio of buffer solution and fly maggot oil is 1:1.0, kept at 40°C, stirred and reacted for 3.5 hours, stopped the reaction, separated at rest, separated the oil phase, washed 5 times with an equal volume of water, and evaporated the oil phase to dryness a

Embodiment 3

[0019] Using the fly maggots cultivated by the breeding method with the patent application number 200510060277.4 as raw materials, wash the fly maggots with water, dry them at a temperature of 35°C at a vacuum of 0.008Mpa until the water content in the fly maggots is 15%, crush them, and add 7 times its weight of A mixed solvent of petroleum ether and hexane, the mixed solvent ratio of petroleum ether and hexane is 1:1, leaching at 20°C for 4 hours, separating the solid and liquid phases, and the liquid phase is evaporated at 40°C under a vacuum of 0.008Mpa to recover petroleum A mixed solvent of ether and hexane to obtain fly maggot oil; in the pH7.0 buffer solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate prepared with water, add bacterial neutral lipase to reach 195 international units of enzyme activity per milliliter; Add fly maggot oil to the buffer solution equipped with bacterial neutral lipase, the volume ratio of buffer solution and fly maggot oil

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PUM

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Abstract

The invention relates to a method for preparing fly oil and relative fatty acid, which comprises that washing the fly water, under 0.01MPa, drying it until the water content of fly is lower than 15%, breaking, adding organic solvent, extracting, separating solid and liquid, recycling the liquid to recycle the solvent to obtain the fly oil; in the buffer liquid prepared from sodium hydrogen phosphate and acid sodium phosphate at 6.0-8.0pH, adding neutral lipase, to reach 180-200 lipase active amount in each milliliter; adding fly oil into the buffer liquid at 1:0.5-1.5 volume ratio, mixing, reacting, stopping reacting, laying and separating, separating the oil, washing with the water in same volume, rotating and drying the oil, to obtain the fly fatty acid. The inventive fly oil and relative fatty acid can be used to treat liver cancer, lung cancer, or the like.

Description

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Claims

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Application Information

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Owner 沈荣法
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