Preparing process and usage of grease of housefly larva and fatty acid thereof
A technology of fly maggot oil and fatty acid, which is applied in the field of bioengineering, can solve the problems of rarely developing health care products, and achieve the effect of not easy to oxidize and deteriorate, easy to absorb, and stable quality
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Embodiment 1
[0015] Using the fly maggots cultivated by the breeding method with the patent application number 200510060277.4 as raw materials, wash the fly maggots with water, dry them at a temperature of 40°C at a vacuum of 0.01Mpa until the water content in the fly maggots is 10%, crush them, and add 1 times its weight Petroleum ether, leached at 0°C for 5 hours, separated solid-liquid two phases, and the liquid phase was evaporated at 20°C under a vacuum of 0.01Mpa to recover petroleum ether to obtain fly maggot oil; In sodium pH6.0 buffer solution, add porcine pancreatic neutral lipase to reach 180 international units of enzyme activity per milliliter; in the buffer solution equipped with porcine pancreatic neutral lipase, add fly maggot oil, the difference between buffer solution and fly maggot oil The volume ratio was 1:0.5, kept at 38°C, stirred and reacted for 1.5 hours, stopped the reaction, separated statically, separated the oil phase, washed twice with an equal volume of water, an
Embodiment 2
[0017] Using the fly maggots cultivated by the breeding method with the patent application number 200510060277.4 as raw materials, wash the fly maggots with water, dry them at a vacuum degree of 0.003Mpa and a temperature of 30°C until the water content in the fly maggots is 13%, crush them, and add 10 times the weight of the fly maggots. Alkanes, leaching at 50°C for 1 hour, separating the solid and liquid phases, and evaporating the liquid phase at 30°C under a vacuum of 0.003Mpa to recover hexane to obtain fly maggot oil; In pH8.0 buffer solution, add yeast neutral lipase, reach per milliliter 200 enzyme activity international units; Add fly maggot oil in the buffer solution that is mixed with yeast neutral lipase, the volume ratio of buffer solution and fly maggot oil is 1:1.0, kept at 40°C, stirred and reacted for 3.5 hours, stopped the reaction, separated at rest, separated the oil phase, washed 5 times with an equal volume of water, and evaporated the oil phase to dryness a
Embodiment 3
[0019] Using the fly maggots cultivated by the breeding method with the patent application number 200510060277.4 as raw materials, wash the fly maggots with water, dry them at a temperature of 35°C at a vacuum of 0.008Mpa until the water content in the fly maggots is 15%, crush them, and add 7 times its weight of A mixed solvent of petroleum ether and hexane, the mixed solvent ratio of petroleum ether and hexane is 1:1, leaching at 20°C for 4 hours, separating the solid and liquid phases, and the liquid phase is evaporated at 40°C under a vacuum of 0.008Mpa to recover petroleum A mixed solvent of ether and hexane to obtain fly maggot oil; in the pH7.0 buffer solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate prepared with water, add bacterial neutral lipase to reach 195 international units of enzyme activity per milliliter; Add fly maggot oil to the buffer solution equipped with bacterial neutral lipase, the volume ratio of buffer solution and fly maggot oil
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