A kind of method for preparing 7-aminocephalosporanic acid

[0024] Example 1

[0025] Add cephalosporin C sodium salt solution to the enzyme reactor, then add D-amino acid oxidase to oxidize, after conversion with hydrogen peroxide, add GL-7 aminocephalosporanic acid acylase to cleave, then crystallize, centrifugally filter, wash, Dry and test to obtain 7-aminocephalosporanic acid with a purity of more than 96%.

[0026] The concentration of the cephalosporin C sodium salt solution is 4%, and its preparation steps: take 48 grams of cephalosporin C sodium salt in a 1200ml beaker, add an appropriate amount of distilled water, and after fully dissolving, adjust the pH to 7.0 with 3N ammonia water About, vacuum filtration to remove insoluble matter, add distilled water to 1200ml, 20 ℃ constant temperature water bath for standby.

[0027] The input amount of the oxidase is 2500u of oxidase per liter of cephalosporin C sodium salt solution.

[0028] The input amount of the acylase is 5000u of acylase per liter of cephalosporin C sodium salt s

[0033] Example 2

[0034] Add cephalosporin C sodium salt solution to the enzyme reactor, then add D-amino acid oxidase to oxidize, after conversion with hydrogen peroxide, add GL-7 aminocephalosporanic acid acylase to cleave, then crystallize, centrifugally filter, wash, Dry and test to obtain 7-aminocephalosporanic acid with a purity of more than 96%.

[0035] The concentration of the cephalosporin C sodium salt solution is 4%, and its preparation steps: take 48 grams of cephalosporin C sodium salt in a 1200ml beaker, add an appropriate amount of distilled water, and after fully dissolving, adjust the pH to 7.0 with 3N ammonia water About, vacuum filtration to remove insoluble matter, add distilled water to 1200ml, 20 ℃ constant temperature water bath for standby.

[0036] The input amount of the oxidase is 2500u of oxidase per liter of cephalosporin C sodium salt solution.

[0037] The input amount of the acylase is 5000u of acylase per liter of cephalosporin C sodium salt s

[0042] Example 3

[0043] 7-Aminocephalosporanic acid double-enzyme conversion HPLC analysis method

[0044] 1. Preparation of mobile phase

[0045] 1. Phase A

[0046] 1.542 g of ammonium acetate was weighed and dissolved in 1000 ml of distilled water, the pH was adjusted to 4.5 with glacial acetic acid, filtered through a 0.45 μm aqueous filter membrane, and degassed for 20 minutes.

[0047] 2. Phase B

[0048] Measure 900ml of phase A, add 100ml of acetonitrile, mix well, and degas for 20min.

[0049] 2. HPLC analysis conditions

[0050] Column type: Rp-8e, 5μm, LiChroCART 250-4

[0051] Total flow rate: 1.0ml / min