Detection method for kidney tonifying and vigour nourishing mixture
A detection method and mixture technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of lack of content determination of Yishenyangyuan mixture, and achieve the effects of high sensitivity, stable baseline and good separation.
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[0021] Example 1 Detection method of Yishen Yangyuan mixture
[0022] HPLC method is used to determine the content of psoralen and isopsoralen in Yishen Yangyuan mixture, including the following steps:
[0023] (1) Prepare the test solution:
[0024] Preparation of sample solution: Take 25ml of Yishenyangyuan mixture and put it in a 100ml volumetric flask, add methanol to the mark, shake, and filter, take 5ml of the filtrate and place it in a 10ml volumetric flask, add mobile phase to the mark, shake it, and get it;
[0025] (2) Prepare the reference solution:
[0026] Accurately weigh the appropriate amount of psoralen and isopsoralen reference substances that have been dried under reduced pressure with phosphorus pentoxide for 24 hours, and dissolve them in methanol to a constant volume to prepare a control containing 10 μg of psoralen and isopsoralen per 1 ml. Product solution
[0027] (3) Determination
[0028] Chromatographic conditions: Chromatographic column is Agilent C18 column (us
Example Embodiment
[0034] Example 2 Conditional screening and methodological verification of a quality analysis method for Yishen Yangyuan mixture
[0035] 1. Selection of extraction solvent
[0036] The solubility of psoralen and isopsoralen in ethanol and methanol is the same, but the sample solution obtained by ethanol dilution is more viscous, impurities and sugar in the sample are more soluble in ethanol than in methanol, so methanol is used as the solvent .
[0037] 2. Selection of detection wavelength
[0038] Take the above-mentioned mixed solution of psoralen and isopsoralen reference substance and scan it on a UV spectrophotometer at a wavelength of 200nm~500nm. The result is that there is a maximum absorption peak at a wavelength of 246nm, so 246nm is used as the detection wavelength.
[0039] 3. Selection of mobile phase
[0040] When the volume ratio of methanol to water reaches 35:65, there is a good separation between the main peak and the impurity peak, and the entire analysis process can be
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