Construction method of fingerprint spectrum of clove and kaki calyx powder as well as standard fingerprint spectrum and application of fingerprint spectrum

[0088] Example 1 Method for constructing a stunned tension fingerprint

[0089] In this example, the construction of the buttam tartary fingerprint is used in the construction of the buttan persimmon.

[0090] Signified 7.46g, persimmon 7.46g, ginseng 3.73g, ginger 11.19g, add 300ml of pure water in the decocting pot, soak 30 minutes, add fried for 10 min, stir with glass rods three times, hot filtration 100 Watching, extrusion filter. The boiling liquid is freeze dry 24 ~ 36h (temperature: -50 ° C, vacuum: 10Pa ~ 20pa), and ground 65 mesh sieve, decenean, Diazi dry powder.

[0091] This example is a method of constructing a stunned tubular fingerprint, including the following specific steps:

[0092] 1) Preparation of the test solution and the reaction solution

[0093] For test solution: Precision weighing 200mg lilac persimmon Tripodiol powder in a 25 mL volumetric flask, adding 25 ml of concentration of 75 wt% methanol aqueous methanol, shake, then extracts 30 min (ultrasound 40

[0115] Example 2 to 5 Construction Method of Type Fingerprint

[0116] Examples 2 to 5 are a construction method of a secluded tension fingerprint, respectively, and their steps are substantially the same as in Example 1, and the difference is only the process parameters. See Table 1 for details.

[0117] Table 1 Example 2 to 5 List of various process parameters

[0118]

[0119] Embodiment 2 to 5 Other parts of the other portions are the same as in Example 1.

[0120] Example 6 Standard fingerprint spectrum of Di'an

[0121] Using Example 1 Construction Method of Dingxia Tommat Scattering Fingerprint, 17 batteries of finished flour, frozen, frozen, frozen, frozen, finish, refreshing graphics, see figure 1 The retention time of each peak in the fingerprints of each batch of diomatommati diazoikin powder is shown in Table 2, and the relative retention time of each peak is shown in Table 3;

[0122] The State Pharmacopoeia Commission "Traditional Chinese Medicine Chromatography Fingerprint Similarity Evaluation System (2004 Edition)" synthesized the above 17 batteries of all-mentioned fingerprint maps Fingerprint, see figure 2 . Among them, there is no food acid in the No. 4 peak as a reference peak, and the relative retention time of its common characteristic peak is:

[0123] No. 1 Peak: 0.354 ~ 0.364, 2 Peak: 0.392 ~ 0.414, 3 Peak: 0.565 ~ 0.574, 4 Peak: 1.000 (S Peak), Peak: 1.542 ~ 1.690, Peak: 1.650 ~ 1.690, 7 peak: 1.796 ~ 1.842, 8 peak