Method for preparing composite enzyme/probiotic preparation for feed by bioconverting rice straw and castor cake pulp

[0026] Example 1

[0027] 1) Mix 10kg of ripe Pu'er tea, 5kg of mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of rice straw, 5kg of castor bean meal, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization, the starter is formed;

[0028] 2) Mix 20kg of starter in step 1) with 600kg of rice straw, 300kg of castor meal, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a thickness of 15cm, natural ventilation, at 40℃ After 4 days of fermentation, a compound enzyme and probiotic preparation for feed is obtained.

[0029] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed of this example are: carboxymethyl cellulase activity reaches 39.65IU / g; total cellulase activity reaches 4.22IU / g; beta-glucosidase activity Reached 9.85IU / g; endoglucanase activity reached 33.54IU / g; xylanase activity reached 38.76IU / g; beta-xylosidase activ

[0030] Example 2

[0031] 1) Mix 10kg of ripe Pu'er tea, 5kg of mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of rice straw, 5kg of castor bean meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization, the starter is formed;

[0032] 2) Mix 20kg of starter in step 1) with 600kg of rice straw, 300kg of castor meal, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a thickness of 15cm, natural ventilation, at 40℃ After 4 days of fermentation, a compound enzyme and probiotic preparation for feed is obtained.

[0033] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed of this example are: carboxymethyl cellulase activity reaches 36.48IU / g; total cellulase activity reaches 4.12IU / g; beta-glucosidase activity Reached 9.56IU / g; endoglucanase activity reached 33.42IU / g; xylanase activity reached 37.68IU / g; beta-xylosidase activ

[0034] Example 3

[0035] 1) Mix 10kg of ripe Pu'er tea, 5kg of mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of rice straw, 5kg of castor bean meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization, the starter is formed;

[0036] 2) Mix 10kg of starter in step 1) with 600kg of rice straw, 300kg of castor meal, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water. Use solid-state fermentation with a thickness of 10cm, natural ventilation, and fermentation at 35°C. In 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed of this example are: carboxymethyl cellulase activity reaches 42.23IU / g; total cellulase activity reaches 4.65IU / g; beta-glucosidase activity Reaching 9.45IU / g; endoglucanase activity reaching 33.25IU / g; xylanase activity reaching 40.43IU / g; beta-xylosidase acti